Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Yan Sun, Gong Zhang, Zizi He, Yajie Wang, Jianlin Cui, Yuhao Li Department of Pathology, Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin, People’s Republic of China Abstract: Copper oxide nanoparticles (CuO NPs) are use...

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Autores principales:	Sun Y, Zhang G, He Z, Wang Y, Cui J, Li Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	copper oxide nanoparticles biotoxicity liver neuronal differentiation zebrafish Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0fe1653bce734279b75fb8bd96f43348
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spelling	oai:doaj.org-article:0fe1653bce734279b75fb8bd96f433482021-12-02T05:36:18ZEffects of copper oxide nanoparticles on developing zebrafish embryos and larvae1178-2013https://doaj.org/article/0fe1653bce734279b75fb8bd96f433482016-03-01T00:00:00Zhttps://www.dovepress.com/effects-of-copper-oxide-nanoparticles-on-developing-zebrafish-embryos--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yan Sun, Gong Zhang, Zizi He, Yajie Wang, Jianlin Cui, Yuhao Li Department of Pathology, Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin, People’s Republic of China Abstract: Copper oxide nanoparticles (CuO NPs) are used for a variety of purposes in a wide range of commercially available products. Some CuO NPs probably end up in the aquatic systems, thus raising concerns about aqueous exposure toxicity, and the impact of CuO NPs on liver development and neuronal differentiation remains unclear. In this study, particles were characterized using Fourier transform infrared spectra, scanning electron microscopy, and transmission electron microscopy. Zebrafish embryos were continuously exposed to CuO NPs from 4 hours postfertilization at concentrations of 50, 25, 12.5, 6.25, or 1 mg/L. The expression of gstp1 and cyp1a was examined by quantitative reverse transcription polymerase chain reaction. The expression of tumor necrosis factor alpha and superoxide dismutase 1 was examined by quantitative reverse transcription polymerase chain reaction and Western blotting. Liver development and retinal neurodifferentiation were analyzed by whole-mount in situ hybridization, hematoxylin–eosin staining, and immunohistochemistry, and a behavioral test was performed to track the movement of larvae. We show that exposure of CuO NPs at low doses has little effect on embryonic development. However, exposure to CuO NPs at concentrations of 12.5 mg/L or higher leads to abnormal phenotypes and induces an inflammatory response in a dose-dependent pattern. Moreover, exposure to CuO NPs at high doses results in an underdeveloped liver and a delay in retinal neurodifferentiation accompanied by reduced locomotor ability. Our data demonstrate that short-term exposure to CuO NPs at high doses shows hepatotoxicity and neurotoxicity in zebrafish embryos and larvae. Keywords: copper oxide nanoparticles, biotoxicity, liver, neuronal differentiation, zebrafish, oxidative stress, behaviorSun YZhang GHe ZWang YCui JLi YDove Medical Pressarticlecopper oxide nanoparticlesbiotoxicityliverneuronal differentiationzebrafishMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 905-918 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	copper oxide nanoparticles biotoxicity liver neuronal differentiation zebrafish Medicine (General) R5-920
spellingShingle	copper oxide nanoparticles biotoxicity liver neuronal differentiation zebrafish Medicine (General) R5-920 Sun Y Zhang G He Z Wang Y Cui J Li Y Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
description	Yan Sun, Gong Zhang, Zizi He, Yajie Wang, Jianlin Cui, Yuhao Li Department of Pathology, Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin, People’s Republic of China Abstract: Copper oxide nanoparticles (CuO NPs) are used for a variety of purposes in a wide range of commercially available products. Some CuO NPs probably end up in the aquatic systems, thus raising concerns about aqueous exposure toxicity, and the impact of CuO NPs on liver development and neuronal differentiation remains unclear. In this study, particles were characterized using Fourier transform infrared spectra, scanning electron microscopy, and transmission electron microscopy. Zebrafish embryos were continuously exposed to CuO NPs from 4 hours postfertilization at concentrations of 50, 25, 12.5, 6.25, or 1 mg/L. The expression of gstp1 and cyp1a was examined by quantitative reverse transcription polymerase chain reaction. The expression of tumor necrosis factor alpha and superoxide dismutase 1 was examined by quantitative reverse transcription polymerase chain reaction and Western blotting. Liver development and retinal neurodifferentiation were analyzed by whole-mount in situ hybridization, hematoxylin–eosin staining, and immunohistochemistry, and a behavioral test was performed to track the movement of larvae. We show that exposure of CuO NPs at low doses has little effect on embryonic development. However, exposure to CuO NPs at concentrations of 12.5 mg/L or higher leads to abnormal phenotypes and induces an inflammatory response in a dose-dependent pattern. Moreover, exposure to CuO NPs at high doses results in an underdeveloped liver and a delay in retinal neurodifferentiation accompanied by reduced locomotor ability. Our data demonstrate that short-term exposure to CuO NPs at high doses shows hepatotoxicity and neurotoxicity in zebrafish embryos and larvae. Keywords: copper oxide nanoparticles, biotoxicity, liver, neuronal differentiation, zebrafish, oxidative stress, behavior
format	article
author	Sun Y Zhang G He Z Wang Y Cui J Li Y
author_facet	Sun Y Zhang G He Z Wang Y Cui J Li Y
author_sort	Sun Y
title	Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
title_short	Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
title_full	Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
title_fullStr	Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
title_full_unstemmed	Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
title_sort	effects of copper oxide nanoparticles on developing zebrafish embryos and larvae
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/0fe1653bce734279b75fb8bd96f43348
work_keys_str_mv	AT suny effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae AT zhangg effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae AT hez effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae AT wangy effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae AT cuij effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae AT liy effectsofcopperoxidenanoparticlesondevelopingzebrafishembryosandlarvae
_version_	1718400276810956800

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

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