Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
Abstract Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents,...
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2021
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oai:doaj.org-article:0fe4241e936348d3a9d34223f627d9ae2021-12-02T18:48:02ZGalvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study10.1038/s41598-021-98451-52045-2322https://doaj.org/article/0fe4241e936348d3a9d34223f627d9ae2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98451-5https://doaj.org/toc/2045-2322Abstract Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly explained by the changes in pH that it generates. However, these effects have not yet been demonstrated. The aim of this study was to evaluate the bactericidal effect and the changes in pH caused by PNE. S. aureus were prepared in two different solutions (TSB and saline solution) and in different concentrations (from 9 to 6 Log10 CFU/mL). Bacteria were treated with three experimental PNE doses to assess bacterial death levels and the changes caused to the pH of the medium. The viable cell count showed that all experimental PNE doses had a bactericidal effect against a high concentration (9 Log10 CFU/mL) of S. aureus in saline solution (p < 0.001). Furthermore, we found that when the concentration of bacteria decreased, a lower dose of galvanic current generated the same effect as a higher dose. Changes in pH were registered only in experiments performed with saline solution. PNE had a bactericidal effect against S. aureus and the level of this effect was mainly modulated by the solution, the bacterial concentration and the dose. Changes affecting pH were modulated by the type of solution and there was no relationship between this and bacterial death.José Antonio García-VidalJesús SalinasPilar Escolar-ReinaFrancisco CuelloNieves OrtegaJuan de Dios Berná-MestreManuel López-NicolásFermín Valera-GarridoFrancesc Medina-MirapeixNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021) |
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Medicine R Science Q |
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Medicine R Science Q José Antonio García-Vidal Jesús Salinas Pilar Escolar-Reina Francisco Cuello Nieves Ortega Juan de Dios Berná-Mestre Manuel López-Nicolás Fermín Valera-Garrido Francesc Medina-Mirapeix Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| description |
Abstract Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly explained by the changes in pH that it generates. However, these effects have not yet been demonstrated. The aim of this study was to evaluate the bactericidal effect and the changes in pH caused by PNE. S. aureus were prepared in two different solutions (TSB and saline solution) and in different concentrations (from 9 to 6 Log10 CFU/mL). Bacteria were treated with three experimental PNE doses to assess bacterial death levels and the changes caused to the pH of the medium. The viable cell count showed that all experimental PNE doses had a bactericidal effect against a high concentration (9 Log10 CFU/mL) of S. aureus in saline solution (p < 0.001). Furthermore, we found that when the concentration of bacteria decreased, a lower dose of galvanic current generated the same effect as a higher dose. Changes in pH were registered only in experiments performed with saline solution. PNE had a bactericidal effect against S. aureus and the level of this effect was mainly modulated by the solution, the bacterial concentration and the dose. Changes affecting pH were modulated by the type of solution and there was no relationship between this and bacterial death. |
| format |
article |
| author |
José Antonio García-Vidal Jesús Salinas Pilar Escolar-Reina Francisco Cuello Nieves Ortega Juan de Dios Berná-Mestre Manuel López-Nicolás Fermín Valera-Garrido Francesc Medina-Mirapeix |
| author_facet |
José Antonio García-Vidal Jesús Salinas Pilar Escolar-Reina Francisco Cuello Nieves Ortega Juan de Dios Berná-Mestre Manuel López-Nicolás Fermín Valera-Garrido Francesc Medina-Mirapeix |
| author_sort |
José Antonio García-Vidal |
| title |
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| title_short |
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| title_full |
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| title_fullStr |
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| title_full_unstemmed |
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| title_sort |
galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/0fe4241e936348d3a9d34223f627d9ae |
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