Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.

Human mesenchymal stem cells (MSCs) are a promising candidate for cell-based transplantation and regenerative medicine therapies. Thus in the present study Wharton's Jelly Mesenchymal Stem Cells (WJ-MSCs) have been derived from extra embryonic umbilical cord matrix following removal of both art...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vikram Sabapathy, Balasubramanian Sundaram, Sreelakshmi V M, Pratheesh Mankuzhy, Sanjay Kumar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0fea61edcc364e728b6410fe56b71398
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0fea61edcc364e728b6410fe56b71398
record_format	dspace
spelling	oai:doaj.org-article:0fea61edcc364e728b6410fe56b713982021-11-18T08:23:14ZHuman Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.1932-620310.1371/journal.pone.0093726https://doaj.org/article/0fea61edcc364e728b6410fe56b713982014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24736473/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human mesenchymal stem cells (MSCs) are a promising candidate for cell-based transplantation and regenerative medicine therapies. Thus in the present study Wharton's Jelly Mesenchymal Stem Cells (WJ-MSCs) have been derived from extra embryonic umbilical cord matrix following removal of both arteries and vein. Also, to overcome the clinical limitations posed by fetal bovine serum (FBS) supplementation because of xenogeneic origin of FBS, usual FBS cell culture supplement has been replaced with human platelet lysate (HPL). Apart from general characteristic features of bone marrow-derived MSCs, wharton jelly-derived MSCs have the ability to maintain phenotypic attributes, cell growth kinetics, cell cycle pattern, in vitro multilineage differentiation plasticity, apoptotic pattern, normal karyotype-like intrinsic mesenchymal stem cell properties in long-term in vitro cultures. Moreover, the WJ-MSCs exhibited the in vitro multilineage differentiation capacity by giving rise to differentiated cells of not only mesodermal lineage but also to the cells of ectodermal and endodermal lineage. Also, WJ-MSC did not present any aberrant cell state upon in vivo transplantation in SCID mice and in vitro soft agar assays. The immunomodulatory potential assessed by gene expression levels of immunomodulatory factors upon exposure to inflammatory cytokines in the fetal WJ-MSCs was relatively higher compared to adult bone marrow-derived MSCs. WJ-MSCs seeded on decellularized amniotic membrane scaffold transplantation on the skin injury of SCID mice model demonstrates that combination of WJ-MSCs and decellularized amniotic membrane scaffold exhibited significantly better wound-healing capabilities, having reduced scar formation with hair growth and improved biomechanical properties of regenerated skin compared to WJ-MSCs alone. Further, our experimental data indicate that indocyanin green (ICG) at optimal concentration can be resourcefully used for labeling of stem cells and in vivo tracking by near infrared fluorescence non-invasive live cell imaging of labelled transplanted cells, thus proving its utility for therapeutic applications.Vikram SabapathyBalasubramanian SundaramSreelakshmi V MPratheesh MankuzhySanjay KumarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93726 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vikram Sabapathy Balasubramanian Sundaram Sreelakshmi V M Pratheesh Mankuzhy Sanjay Kumar Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
description	Human mesenchymal stem cells (MSCs) are a promising candidate for cell-based transplantation and regenerative medicine therapies. Thus in the present study Wharton's Jelly Mesenchymal Stem Cells (WJ-MSCs) have been derived from extra embryonic umbilical cord matrix following removal of both arteries and vein. Also, to overcome the clinical limitations posed by fetal bovine serum (FBS) supplementation because of xenogeneic origin of FBS, usual FBS cell culture supplement has been replaced with human platelet lysate (HPL). Apart from general characteristic features of bone marrow-derived MSCs, wharton jelly-derived MSCs have the ability to maintain phenotypic attributes, cell growth kinetics, cell cycle pattern, in vitro multilineage differentiation plasticity, apoptotic pattern, normal karyotype-like intrinsic mesenchymal stem cell properties in long-term in vitro cultures. Moreover, the WJ-MSCs exhibited the in vitro multilineage differentiation capacity by giving rise to differentiated cells of not only mesodermal lineage but also to the cells of ectodermal and endodermal lineage. Also, WJ-MSC did not present any aberrant cell state upon in vivo transplantation in SCID mice and in vitro soft agar assays. The immunomodulatory potential assessed by gene expression levels of immunomodulatory factors upon exposure to inflammatory cytokines in the fetal WJ-MSCs was relatively higher compared to adult bone marrow-derived MSCs. WJ-MSCs seeded on decellularized amniotic membrane scaffold transplantation on the skin injury of SCID mice model demonstrates that combination of WJ-MSCs and decellularized amniotic membrane scaffold exhibited significantly better wound-healing capabilities, having reduced scar formation with hair growth and improved biomechanical properties of regenerated skin compared to WJ-MSCs alone. Further, our experimental data indicate that indocyanin green (ICG) at optimal concentration can be resourcefully used for labeling of stem cells and in vivo tracking by near infrared fluorescence non-invasive live cell imaging of labelled transplanted cells, thus proving its utility for therapeutic applications.
format	article
author	Vikram Sabapathy Balasubramanian Sundaram Sreelakshmi V M Pratheesh Mankuzhy Sanjay Kumar
author_facet	Vikram Sabapathy Balasubramanian Sundaram Sreelakshmi V M Pratheesh Mankuzhy Sanjay Kumar
author_sort	Vikram Sabapathy
title	Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
title_short	Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
title_full	Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
title_fullStr	Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
title_full_unstemmed	Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.
title_sort	human wharton's jelly mesenchymal stem cells plasticity augments scar-free skin wound healing with hair growth.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0fea61edcc364e728b6410fe56b71398
work_keys_str_mv	AT vikramsabapathy humanwhartonsjellymesenchymalstemcellsplasticityaugmentsscarfreeskinwoundhealingwithhairgrowth AT balasubramaniansundaram humanwhartonsjellymesenchymalstemcellsplasticityaugmentsscarfreeskinwoundhealingwithhairgrowth AT sreelakshmivm humanwhartonsjellymesenchymalstemcellsplasticityaugmentsscarfreeskinwoundhealingwithhairgrowth AT pratheeshmankuzhy humanwhartonsjellymesenchymalstemcellsplasticityaugmentsscarfreeskinwoundhealingwithhairgrowth AT sanjaykumar humanwhartonsjellymesenchymalstemcellsplasticityaugmentsscarfreeskinwoundhealingwithhairgrowth
_version_	1718421844169588736

Human Wharton's Jelly Mesenchymal Stem Cells plasticity augments scar-free skin wound healing with hair growth.

Ejemplares similares