Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair

ABSTRACT The mating efficiency of 50 Aspergillus fumigatus isolates from both clinical and environmental sources was analyzed. Forty isolates completed the sexual cycle in 4 weeks with variable levels of fertility designated high, medium, or low. Two opposite-mating-type strains exhibiting the highe...

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Autores principales:	Janyce A. Sugui, Liliana Losada, Wei Wang, John Varga, Popchai Ngamskulrungroj, Mones Abu-Asab, Yun C. Chang, Céline M. O’Gorman, Brian L. Wickes, William C. Nierman, Paul S. Dyer, Kyung J. Kwon-Chung
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2011
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0feffac24d98427497b4966e7ac0bd35
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spelling	oai:doaj.org-article:0feffac24d98427497b4966e7ac0bd352021-11-15T15:38:49ZIdentification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair10.1128/mBio.00234-112150-7511https://doaj.org/article/0feffac24d98427497b4966e7ac0bd352011-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00234-11https://doaj.org/toc/2150-7511ABSTRACT The mating efficiency of 50 Aspergillus fumigatus isolates from both clinical and environmental sources was analyzed. Forty isolates completed the sexual cycle in 4 weeks with variable levels of fertility designated high, medium, or low. Two opposite-mating-type strains exhibiting the highest fertility, AFB62 (MAT1-1), isolated from a case of invasive aspergillosis, and AFIR928 (MAT1-2), isolated from the environment, were chosen as the supermater pair. Single cleistothecia obtained from a cross of the two strains harbored a minimum of 1 × 104 ascospores. The viability of ascospores increased with the age of the fruiting body, 17% at 4 weeks and reaching 95% at 20 weeks. AFB62 and AFIR928 were equally virulent in two different murine models, despite differences in their sources. High recombination frequencies were observed when the closely linked genes alb1 (AFUA_2G17600) and abr2 (AFUA_2G17530) were used as genetic markers. Comparative genome hybridization analyses revealed that only 86 genes (ca. 0.86% of the genome) are significantly diverged between AFB62 and AFIR928. The high fertility in a relatively short period, combined with a high degree of virulence and a high recombination frequency, demonstrates that the mating pair AFB62 and AFIR928 provides an excellent tool for genetic studies of A. fumigatus. IMPORTANCE Aspergillus fumigatus is a heterothallic fungal pathogen that causes life-threatening infections in immunocompromised hosts. Although heterothallism facilitates genetic study via recombinational analysis, previous work showed that a 6-month incubation period is required for the completion of sexual reproduction in this species. Such a long incubation period impedes progress in genetic research. To discover a highly fertile (supermater) pair that can complete the sexual cycle in a considerably shorter period, we screened 50 strains collected from various geographic regions for mating efficiency. We identified a highly virulent pair of supermaters that can be an invaluable tool for genetic study.Janyce A. SuguiLiliana LosadaWei WangJohn VargaPopchai NgamskulrungrojMones Abu-AsabYun C. ChangCéline M. O’GormanBrian L. WickesWilliam C. NiermanPaul S. DyerKyung J. Kwon-ChungAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 2, Iss 6 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Janyce A. Sugui Liliana Losada Wei Wang John Varga Popchai Ngamskulrungroj Mones Abu-Asab Yun C. Chang Céline M. O’Gorman Brian L. Wickes William C. Nierman Paul S. Dyer Kyung J. Kwon-Chung Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
description	ABSTRACT The mating efficiency of 50 Aspergillus fumigatus isolates from both clinical and environmental sources was analyzed. Forty isolates completed the sexual cycle in 4 weeks with variable levels of fertility designated high, medium, or low. Two opposite-mating-type strains exhibiting the highest fertility, AFB62 (MAT1-1), isolated from a case of invasive aspergillosis, and AFIR928 (MAT1-2), isolated from the environment, were chosen as the supermater pair. Single cleistothecia obtained from a cross of the two strains harbored a minimum of 1 × 104 ascospores. The viability of ascospores increased with the age of the fruiting body, 17% at 4 weeks and reaching 95% at 20 weeks. AFB62 and AFIR928 were equally virulent in two different murine models, despite differences in their sources. High recombination frequencies were observed when the closely linked genes alb1 (AFUA_2G17600) and abr2 (AFUA_2G17530) were used as genetic markers. Comparative genome hybridization analyses revealed that only 86 genes (ca. 0.86% of the genome) are significantly diverged between AFB62 and AFIR928. The high fertility in a relatively short period, combined with a high degree of virulence and a high recombination frequency, demonstrates that the mating pair AFB62 and AFIR928 provides an excellent tool for genetic studies of A. fumigatus. IMPORTANCE Aspergillus fumigatus is a heterothallic fungal pathogen that causes life-threatening infections in immunocompromised hosts. Although heterothallism facilitates genetic study via recombinational analysis, previous work showed that a 6-month incubation period is required for the completion of sexual reproduction in this species. Such a long incubation period impedes progress in genetic research. To discover a highly fertile (supermater) pair that can complete the sexual cycle in a considerably shorter period, we screened 50 strains collected from various geographic regions for mating efficiency. We identified a highly virulent pair of supermaters that can be an invaluable tool for genetic study.
format	article
author	Janyce A. Sugui Liliana Losada Wei Wang John Varga Popchai Ngamskulrungroj Mones Abu-Asab Yun C. Chang Céline M. O’Gorman Brian L. Wickes William C. Nierman Paul S. Dyer Kyung J. Kwon-Chung
author_facet	Janyce A. Sugui Liliana Losada Wei Wang John Varga Popchai Ngamskulrungroj Mones Abu-Asab Yun C. Chang Céline M. O’Gorman Brian L. Wickes William C. Nierman Paul S. Dyer Kyung J. Kwon-Chung
author_sort	Janyce A. Sugui
title	Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
title_short	Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
title_full	Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
title_fullStr	Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
title_full_unstemmed	Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair
title_sort	identification and characterization of an <named-content content-type="genus-species">aspergillus fumigatus</named-content> “supermater” pair
publisher	American Society for Microbiology
publishDate	2011
url	https://doaj.org/article/0feffac24d98427497b4966e7ac0bd35
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Identification and Characterization of an <named-content content-type="genus-species">Aspergillus fumigatus</named-content> “Supermater” Pair

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