Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes

The chronic irreversible regression of cognitive ability and memory function in human immunodeficiency virus (HIV)-associated dementia (HAND) is linked with late-stage HIV infection in the brain. The molecular-level signatures of neuroinflammation and neurodegeneration are linked with dysfunction in...

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Autores principales:	Mayur Doke, Tamizhselvi Ramasamy, Vaishnavi Sundar, Jay P. McLaughlin, Thangavel Samikkannu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	proteomics SWATH HIV-associated dementia frontal lobe human brain LC-MS/MS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/0ff0bf98961a4dcea3b62196442b2f07
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spelling	oai:doaj.org-article:0ff0bf98961a4dcea3b62196442b2f072021-11-25T16:57:24ZProteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes10.3390/brainsci111114382076-3425https://doaj.org/article/0ff0bf98961a4dcea3b62196442b2f072021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3425/11/11/1438https://doaj.org/toc/2076-3425The chronic irreversible regression of cognitive ability and memory function in human immunodeficiency virus (HIV)-associated dementia (HAND) is linked with late-stage HIV infection in the brain. The molecular-level signatures of neuroinflammation and neurodegeneration are linked with dysfunction in HAND patients. Protein expression changes and posttranslational modification are epigenetic cues for dementia and neurodegenerative disease. In this study quantitative proteome analysis was performed to comprehensively elucidate changes in protein profiles in HIV-positive (HIV+) human brains. Frontal and temporal lobes of normal and HIV+ brains were subjected to label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the data-independent acquisition method. Comprehensive proteomic identification and quantification analysis revealed that 3294 total proteins and 251 proteins were differentially expressed in HIV+ brains; specifically, HIV+ frontal and temporal lobes had 132 and 119 differentially expressed proteins, respectively. Proteomic and bioinformatic analyses revealed protein alterations predominantly in the HIV+ frontal lobe region. The expression of GOLPH3, IMPDH2, DYNLL1, RPL11, and GPNMB proteins was significantly altered in HIV+ frontal lobes compared to that in normal brains. These proteins are associated with metabolic pathways, neurodegenerative disorders, and dementia. These proteomic-level changes may be potential biological markers and therapeutic targets to relieve the dementia-associated symptoms in individuals with HAND.Mayur DokeTamizhselvi RamasamyVaishnavi SundarJay P. McLaughlinThangavel SamikkannuMDPI AGarticleproteomicsSWATHHIV-associated dementiafrontal lobehuman brainLC-MS/MSNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBrain Sciences, Vol 11, Iss 1438, p 1438 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proteomics SWATH HIV-associated dementia frontal lobe human brain LC-MS/MS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	proteomics SWATH HIV-associated dementia frontal lobe human brain LC-MS/MS Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Mayur Doke Tamizhselvi Ramasamy Vaishnavi Sundar Jay P. McLaughlin Thangavel Samikkannu Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
description	The chronic irreversible regression of cognitive ability and memory function in human immunodeficiency virus (HIV)-associated dementia (HAND) is linked with late-stage HIV infection in the brain. The molecular-level signatures of neuroinflammation and neurodegeneration are linked with dysfunction in HAND patients. Protein expression changes and posttranslational modification are epigenetic cues for dementia and neurodegenerative disease. In this study quantitative proteome analysis was performed to comprehensively elucidate changes in protein profiles in HIV-positive (HIV+) human brains. Frontal and temporal lobes of normal and HIV+ brains were subjected to label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the data-independent acquisition method. Comprehensive proteomic identification and quantification analysis revealed that 3294 total proteins and 251 proteins were differentially expressed in HIV+ brains; specifically, HIV+ frontal and temporal lobes had 132 and 119 differentially expressed proteins, respectively. Proteomic and bioinformatic analyses revealed protein alterations predominantly in the HIV+ frontal lobe region. The expression of GOLPH3, IMPDH2, DYNLL1, RPL11, and GPNMB proteins was significantly altered in HIV+ frontal lobes compared to that in normal brains. These proteins are associated with metabolic pathways, neurodegenerative disorders, and dementia. These proteomic-level changes may be potential biological markers and therapeutic targets to relieve the dementia-associated symptoms in individuals with HAND.
format	article
author	Mayur Doke Tamizhselvi Ramasamy Vaishnavi Sundar Jay P. McLaughlin Thangavel Samikkannu
author_facet	Mayur Doke Tamizhselvi Ramasamy Vaishnavi Sundar Jay P. McLaughlin Thangavel Samikkannu
author_sort	Mayur Doke
title	Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
title_short	Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
title_full	Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
title_fullStr	Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
title_full_unstemmed	Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes
title_sort	proteomics profiling with swath-ms quantitative analysis of changes in the human brain with hiv infection reveals a differential impact on the frontal and temporal lobes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0ff0bf98961a4dcea3b62196442b2f07
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Proteomics Profiling with SWATH-MS Quantitative Analysis of Changes in the Human Brain with HIV Infection Reveals a Differential Impact on the Frontal and Temporal Lobes

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