A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin

Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified ligands in complex with tubulin.

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Autores principales:	Grégory Menchon, Andrea E. Prota, Daniel Lucena-Agell, Pascal Bucher, Rolf Jansen, Herbert Irschik, Rolf Müller, Ian Paterson, J. Fernando Díaz, Karl-Heinz Altmann, Michel O. Steinmetz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0ff5964567e04f11ab1504ca1c9d29cf
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spelling	oai:doaj.org-article:0ff5964567e04f11ab1504ca1c9d29cf2021-12-02T14:39:46ZA fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin10.1038/s41467-018-04535-82041-1723https://doaj.org/article/0ff5964567e04f11ab1504ca1c9d29cf2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04535-8https://doaj.org/toc/2041-1723Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified ligands in complex with tubulin.Grégory MenchonAndrea E. ProtaDaniel Lucena-AgellPascal BucherRolf JansenHerbert IrschikRolf MüllerIan PatersonJ. Fernando DíazKarl-Heinz AltmannMichel O. SteinmetzNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Grégory Menchon Andrea E. Prota Daniel Lucena-Agell Pascal Bucher Rolf Jansen Herbert Irschik Rolf Müller Ian Paterson J. Fernando Díaz Karl-Heinz Altmann Michel O. Steinmetz A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
description	Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified ligands in complex with tubulin.
format	article
author	Grégory Menchon Andrea E. Prota Daniel Lucena-Agell Pascal Bucher Rolf Jansen Herbert Irschik Rolf Müller Ian Paterson J. Fernando Díaz Karl-Heinz Altmann Michel O. Steinmetz
author_facet	Grégory Menchon Andrea E. Prota Daniel Lucena-Agell Pascal Bucher Rolf Jansen Herbert Irschik Rolf Müller Ian Paterson J. Fernando Díaz Karl-Heinz Altmann Michel O. Steinmetz
author_sort	Grégory Menchon
title	A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
title_short	A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
title_full	A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
title_fullStr	A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
title_full_unstemmed	A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
title_sort	fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0ff5964567e04f11ab1504ca1c9d29cf
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A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin

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