New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization

Roberta Cavalli,1 Agnese Bisazza,1 Michele Trotta,1 Monica Argenziano,1 Andrea Civra,2 Manuela Donalisio,2 David Lembo21Department of Pharmaceutical Sciences and Technology; 2Department of Clinical and Biological Sciences University of Turin, Turin, ItalyBackground: The development of nonviral gene...

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Autores principales:	Cavalli R, Bisazza A, Trotta M, Argenziano M, Civra A, Donalisio M, Lembo D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/10192dca53784ceb85c621a6ee5047f3
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spelling	oai:doaj.org-article:10192dca53784ceb85c621a6ee5047f32021-12-02T01:13:36ZNew chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization1176-91141178-2013https://doaj.org/article/10192dca53784ceb85c621a6ee5047f32012-06-01T00:00:00Zhttp://www.dovepress.com/new-chitosan-nanobubbles-for-ultrasound-mediated-gene-delivery-prepara-a10254https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Roberta Cavalli,1 Agnese Bisazza,1 Michele Trotta,1 Monica Argenziano,1 Andrea Civra,2 Manuela Donalisio,2 David Lembo21Department of Pharmaceutical Sciences and Technology; 2Department of Clinical and Biological Sciences University of Turin, Turin, ItalyBackground: The development of nonviral gene delivery systems is one of the most intriguing topics in nanomedicine. However, despite the advances made in recent years, several key issues remain unsettled. One of the main problems relates to the difficulty in designing nanodevices for targeted delivery of genes and other drugs to specific anatomic sites. In this study, we describe the development of a novel chitosan nanobubble-based gene delivery system for ultrasound-triggered release.Methods and results: Chitosan was selected for the nanobubble shell because of its low toxicity, low immunogenicity, and excellent biocompatibility, while the core consisted of perfluoropentane. DNA-loaded chitosan nanobubbles were formed with a mean diameter of less than 300 nm and a positive surface charge. Transmission electron microscopic analysis confirmed composition of the core-shell structure. The ability of the chitosan nanobubbles to complex with and protect DNA was confirmed by agarose gel assay. Chitosan nanobubbles were found to be stable following insonation (2.5 MHz) for up to 3 minutes at 37&deg;C. DNA release was evaluated in vitro in both the presence and absence of ultrasound. The release of chitosan nanobubble-bound plasmid DNA occurred after just one minute of insonation. In vitro transfection experiments were performed by exposing adherent COS7 cells to ultrasound in the presence of different concentrations of plasmid DNA-loaded nanobubbles. In the absence of ultrasound, nanobubbles failed to trigger transfection at all concentrations tested. In contrast, 30 seconds of ultrasound promoted a moderate degree of transfection. Cell viability experiments demonstrated that neither ultrasound nor the nanobubbles affected cell viability under these experimental conditions.Conclusion: Based on these results, chitosan nanobubbles have the potential to be promising tools for ultrasound-mediated DNA delivery.Keywords: chitosan, nanobubbles, transfection, DNA, gene carrier, ultrasoundCavalli RBisazza ATrotta MArgenziano MCivra ADonalisio MLembo DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3309-3318 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cavalli R Bisazza A Trotta M Argenziano M Civra A Donalisio M Lembo D New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
description	Roberta Cavalli,1 Agnese Bisazza,1 Michele Trotta,1 Monica Argenziano,1 Andrea Civra,2 Manuela Donalisio,2 David Lembo21Department of Pharmaceutical Sciences and Technology; 2Department of Clinical and Biological Sciences University of Turin, Turin, ItalyBackground: The development of nonviral gene delivery systems is one of the most intriguing topics in nanomedicine. However, despite the advances made in recent years, several key issues remain unsettled. One of the main problems relates to the difficulty in designing nanodevices for targeted delivery of genes and other drugs to specific anatomic sites. In this study, we describe the development of a novel chitosan nanobubble-based gene delivery system for ultrasound-triggered release.Methods and results: Chitosan was selected for the nanobubble shell because of its low toxicity, low immunogenicity, and excellent biocompatibility, while the core consisted of perfluoropentane. DNA-loaded chitosan nanobubbles were formed with a mean diameter of less than 300 nm and a positive surface charge. Transmission electron microscopic analysis confirmed composition of the core-shell structure. The ability of the chitosan nanobubbles to complex with and protect DNA was confirmed by agarose gel assay. Chitosan nanobubbles were found to be stable following insonation (2.5 MHz) for up to 3 minutes at 37&deg;C. DNA release was evaluated in vitro in both the presence and absence of ultrasound. The release of chitosan nanobubble-bound plasmid DNA occurred after just one minute of insonation. In vitro transfection experiments were performed by exposing adherent COS7 cells to ultrasound in the presence of different concentrations of plasmid DNA-loaded nanobubbles. In the absence of ultrasound, nanobubbles failed to trigger transfection at all concentrations tested. In contrast, 30 seconds of ultrasound promoted a moderate degree of transfection. Cell viability experiments demonstrated that neither ultrasound nor the nanobubbles affected cell viability under these experimental conditions.Conclusion: Based on these results, chitosan nanobubbles have the potential to be promising tools for ultrasound-mediated DNA delivery.Keywords: chitosan, nanobubbles, transfection, DNA, gene carrier, ultrasound
format	article
author	Cavalli R Bisazza A Trotta M Argenziano M Civra A Donalisio M Lembo D
author_facet	Cavalli R Bisazza A Trotta M Argenziano M Civra A Donalisio M Lembo D
author_sort	Cavalli R
title	New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
title_short	New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
title_full	New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
title_fullStr	New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
title_full_unstemmed	New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
title_sort	new chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/10192dca53784ceb85c621a6ee5047f3
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New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization

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