Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8
Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process param...
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2021
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oai:doaj.org-article:10224199e8704d3a9367e2d9cb104f9e2021-11-25T18:51:54ZBiodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS810.3390/pr91120642227-9717https://doaj.org/article/10224199e8704d3a9367e2d9cb104f9e2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2064https://doaj.org/toc/2227-9717Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), and type of sulfur compound—namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT), and 4,6-diethyldibenzothiophene (4,6-DEDBT)—were evaluated, using resting cells of <i>Rhodococcus erythropolis</i> IGTS8. Cells derived from the beginning of the exponential growth phase of the bacterium exhibited the highest biodesulfurization efficiency and rate. The biocatalyst performed better in an OFP of 50% <i>v</i>/<i>v</i>. The extent of DBT desulfurization was dependent on cell concentration, with the desulfurization rate reaching its maximum at intermediate cell concentrations. A new semi-empirical model for the biphasic BDS was developed, based on the overall Michaelis-Menten kinetics and taking into consideration the deactivation of the biocatalyst over time, as well as the underlying mass transfer phenomena. The model fitted experimental data on DBT consumption and 2-hydroxibyphenyl (2-HBP) accumulation in the organic phase for various initial DBT concentrations and different organosulfur compounds. For constant OFP and biocatalyst concentration, the most important parameter that affects BDS efficiency seems to be biocatalyst deactivation, while the phenomenon is controlled by the affinities of biodesulfurizing enzymes for the different organosulfur compounds. Thus, desulfurization efficiency decreased with increasing initial DBT concentration, and in inverse proportion to increases in the carbon number of alkyl substituent groups.George PrasoulasKonstantinos DimosPanayiotis GlekasStyliani KalantziStamatis SarrisChrysovalantis TemplisKonstantinos VavitsasDimitris G. HatzinikolaouNikolaos PapayannakosDimitris KekosDiomi MammaMDPI AGarticlebiodesulfurization kinetics<i>Rhodococcus erythropolis</i> IGTS8resting cellsdibenzothiophenealkylated dibenzothiophenebubble column bioreactorChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2064, p 2064 (2021) |
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DOAJ |
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biodesulfurization kinetics <i>Rhodococcus erythropolis</i> IGTS8 resting cells dibenzothiophene alkylated dibenzothiophene bubble column bioreactor Chemical technology TP1-1185 Chemistry QD1-999 |
| spellingShingle |
biodesulfurization kinetics <i>Rhodococcus erythropolis</i> IGTS8 resting cells dibenzothiophene alkylated dibenzothiophene bubble column bioreactor Chemical technology TP1-1185 Chemistry QD1-999 George Prasoulas Konstantinos Dimos Panayiotis Glekas Styliani Kalantzi Stamatis Sarris Chrysovalantis Templis Konstantinos Vavitsas Dimitris G. Hatzinikolaou Nikolaos Papayannakos Dimitris Kekos Diomi Mamma Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| description |
Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), and type of sulfur compound—namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT), and 4,6-diethyldibenzothiophene (4,6-DEDBT)—were evaluated, using resting cells of <i>Rhodococcus erythropolis</i> IGTS8. Cells derived from the beginning of the exponential growth phase of the bacterium exhibited the highest biodesulfurization efficiency and rate. The biocatalyst performed better in an OFP of 50% <i>v</i>/<i>v</i>. The extent of DBT desulfurization was dependent on cell concentration, with the desulfurization rate reaching its maximum at intermediate cell concentrations. A new semi-empirical model for the biphasic BDS was developed, based on the overall Michaelis-Menten kinetics and taking into consideration the deactivation of the biocatalyst over time, as well as the underlying mass transfer phenomena. The model fitted experimental data on DBT consumption and 2-hydroxibyphenyl (2-HBP) accumulation in the organic phase for various initial DBT concentrations and different organosulfur compounds. For constant OFP and biocatalyst concentration, the most important parameter that affects BDS efficiency seems to be biocatalyst deactivation, while the phenomenon is controlled by the affinities of biodesulfurizing enzymes for the different organosulfur compounds. Thus, desulfurization efficiency decreased with increasing initial DBT concentration, and in inverse proportion to increases in the carbon number of alkyl substituent groups. |
| format |
article |
| author |
George Prasoulas Konstantinos Dimos Panayiotis Glekas Styliani Kalantzi Stamatis Sarris Chrysovalantis Templis Konstantinos Vavitsas Dimitris G. Hatzinikolaou Nikolaos Papayannakos Dimitris Kekos Diomi Mamma |
| author_facet |
George Prasoulas Konstantinos Dimos Panayiotis Glekas Styliani Kalantzi Stamatis Sarris Chrysovalantis Templis Konstantinos Vavitsas Dimitris G. Hatzinikolaou Nikolaos Papayannakos Dimitris Kekos Diomi Mamma |
| author_sort |
George Prasoulas |
| title |
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| title_short |
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| title_full |
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| title_fullStr |
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| title_full_unstemmed |
Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of <i>Rhodococcus erythropolis</i> IGTS8 |
| title_sort |
biodesulfurization of dibenzothiophene and its alkylated derivatives in a two-phase bubble column bioreactor by resting cells of <i>rhodococcus erythropolis</i> igts8 |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/10224199e8704d3a9367e2d9cb104f9e |
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