Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation

This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both form...

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Autores principales:	Salima El Moussaoui, Ismael Abo-Horan, Lyda Halbaut, Cristina Alonso, Lluïsa Coderch, María Luisa Garduño-Ramírez, Beatriz Clares, José Luis Soriano, Ana Cristina Calpena, Francisco Fernández-Campos, Mireia Mallandrich
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PLGA nanoparticles chitosan gel Condyloma acuminata ketorolac tromethamine anti-inflammatory topical delivery Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/1028904f959e4354a2ce2959a684b11c
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spelling	oai:doaj.org-article:1028904f959e4354a2ce2959a684b11c2021-11-25T18:40:39ZPolymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation10.3390/pharmaceutics131117841999-4923https://doaj.org/article/1028904f959e4354a2ce2959a684b11c2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1784https://doaj.org/toc/1999-4923This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application.Salima El MoussaouiIsmael Abo-HoranLyda HalbautCristina AlonsoLluïsa CoderchMaría Luisa Garduño-RamírezBeatriz ClaresJosé Luis SorianoAna Cristina CalpenaFrancisco Fernández-CamposMireia MallandrichMDPI AGarticlePLGA nanoparticleschitosan gelCondyloma acuminataketorolac tromethamineanti-inflammatorytopical deliveryPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1784, p 1784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PLGA nanoparticles chitosan gel Condyloma acuminata ketorolac tromethamine anti-inflammatory topical delivery Pharmacy and materia medica RS1-441
spellingShingle	PLGA nanoparticles chitosan gel Condyloma acuminata ketorolac tromethamine anti-inflammatory topical delivery Pharmacy and materia medica RS1-441 Salima El Moussaoui Ismael Abo-Horan Lyda Halbaut Cristina Alonso Lluïsa Coderch María Luisa Garduño-Ramírez Beatriz Clares José Luis Soriano Ana Cristina Calpena Francisco Fernández-Campos Mireia Mallandrich Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
description	This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application.
format	article
author	Salima El Moussaoui Ismael Abo-Horan Lyda Halbaut Cristina Alonso Lluïsa Coderch María Luisa Garduño-Ramírez Beatriz Clares José Luis Soriano Ana Cristina Calpena Francisco Fernández-Campos Mireia Mallandrich
author_facet	Salima El Moussaoui Ismael Abo-Horan Lyda Halbaut Cristina Alonso Lluïsa Coderch María Luisa Garduño-Ramírez Beatriz Clares José Luis Soriano Ana Cristina Calpena Francisco Fernández-Campos Mireia Mallandrich
author_sort	Salima El Moussaoui
title	Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_short	Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full	Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_fullStr	Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full_unstemmed	Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_sort	polymeric nanoparticles and chitosan gel loading ketorolac tromethamine to alleviate pain associated with condyloma acuminata during the pre- and post-ablation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1028904f959e4354a2ce2959a684b11c
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Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation

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