Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn

Abstract Burn wound progression is an important determinant of patient morbidity and mortality after injury. In this study, we used the brass comb contact burn to determine burn wound vertical injury progression with a focus on blood vessel occlusion and endothelial cell death. Class A 3-month-old Y...

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Autores principales:	Jing Wang, Cheng Z. Wang, John R. Salsbury, Jianzi Zhang, Perenlei Enkhbaatar, David N. Herndon, Amina El Ayadi, Naseem H. Ansari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1028ae025be443d5adf3f624d995332d
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spelling	oai:doaj.org-article:1028ae025be443d5adf3f624d995332d2021-12-02T17:24:22ZThermal injury induces early blood vessel occlusion in a porcine model of brass comb burn10.1038/s41598-021-91874-02045-2322https://doaj.org/article/1028ae025be443d5adf3f624d995332d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91874-0https://doaj.org/toc/2045-2322Abstract Burn wound progression is an important determinant of patient morbidity and mortality after injury. In this study, we used the brass comb contact burn to determine burn wound vertical injury progression with a focus on blood vessel occlusion and endothelial cell death. Class A 3-month-old Yorkshire pigs received a brass comb contact burn. Burn wounds were sampled at 0, 30 min, 1, 2, 4, and 24 h. Hematoxylin Phloxin Saffron staining and vimentin immunostaining were performed to determine the depth of blood vessel occlusion and endothelial cell death, respectively. The depth of blood vessel occlusion increased by 30 min (p < 0.005) and peaked by 1 to 4 h (p > 0.05). The depth of endothelial cell death risen to a plateau at 30 min (p < 0.005) to 2 h and then peaked at 24 h (p < 0.03). We observed a progression of blood vessel occlusion and vascular endothelial cell death from the middle of the dermis to the hypodermis within 2 h to 4 h after the initial injury, namely a progression from a second-degree (partial thickness) to third-degree (full thickness) burn. These data suggest that therapeutic interventions during this time window may provide a better outcome by reducing or preventing vertical progression of blood vascular occlusion or endothelial cell death.Jing WangCheng Z. WangJohn R. SalsburyJianzi ZhangPerenlei EnkhbaatarDavid N. HerndonAmina El AyadiNaseem H. AnsariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Wang Cheng Z. Wang John R. Salsbury Jianzi Zhang Perenlei Enkhbaatar David N. Herndon Amina El Ayadi Naseem H. Ansari Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
description	Abstract Burn wound progression is an important determinant of patient morbidity and mortality after injury. In this study, we used the brass comb contact burn to determine burn wound vertical injury progression with a focus on blood vessel occlusion and endothelial cell death. Class A 3-month-old Yorkshire pigs received a brass comb contact burn. Burn wounds were sampled at 0, 30 min, 1, 2, 4, and 24 h. Hematoxylin Phloxin Saffron staining and vimentin immunostaining were performed to determine the depth of blood vessel occlusion and endothelial cell death, respectively. The depth of blood vessel occlusion increased by 30 min (p < 0.005) and peaked by 1 to 4 h (p > 0.05). The depth of endothelial cell death risen to a plateau at 30 min (p < 0.005) to 2 h and then peaked at 24 h (p < 0.03). We observed a progression of blood vessel occlusion and vascular endothelial cell death from the middle of the dermis to the hypodermis within 2 h to 4 h after the initial injury, namely a progression from a second-degree (partial thickness) to third-degree (full thickness) burn. These data suggest that therapeutic interventions during this time window may provide a better outcome by reducing or preventing vertical progression of blood vascular occlusion or endothelial cell death.
format	article
author	Jing Wang Cheng Z. Wang John R. Salsbury Jianzi Zhang Perenlei Enkhbaatar David N. Herndon Amina El Ayadi Naseem H. Ansari
author_facet	Jing Wang Cheng Z. Wang John R. Salsbury Jianzi Zhang Perenlei Enkhbaatar David N. Herndon Amina El Ayadi Naseem H. Ansari
author_sort	Jing Wang
title	Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
title_short	Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
title_full	Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
title_fullStr	Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
title_full_unstemmed	Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
title_sort	thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1028ae025be443d5adf3f624d995332d
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Thermal injury induces early blood vessel occlusion in a porcine model of brass comb burn

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