The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo

Cow mastitis, which significantly lowers milk quality, is mainly caused by pathogenic bacteria such as E. coli. Previous studies have suggested that lactic acid bacteria can have antagonistic effects on pathogenic bacteria that cause mastitis. In the current study, we evaluated the in vitro and in v...

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Autores principales:	Qingxue Chen, Song Wang, Jiayao Guo, Qinggang Xie, Smith Etareri Evivie, Yue Song, Bailiang Li, Guicheng Huo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Lactobacillus plantarum mastitis antibacterial immunomodulation signal path Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1028dad9d1d946c282578545b2a36389
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spelling	oai:doaj.org-article:1028dad9d1d946c282578545b2a363892021-11-09T06:15:57ZThe Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo1664-322410.3389/fimmu.2021.770822https://doaj.org/article/1028dad9d1d946c282578545b2a363892021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.770822/fullhttps://doaj.org/toc/1664-3224Cow mastitis, which significantly lowers milk quality, is mainly caused by pathogenic bacteria such as E. coli. Previous studies have suggested that lactic acid bacteria can have antagonistic effects on pathogenic bacteria that cause mastitis. In the current study, we evaluated the in vitro and in vivo alleviative effects of L. plantarum KLDS 1.0344 in mastitis treatment. In vitro antibacterial experiments were performed using bovine mammary epithelial cell (bMEC), followed by in vivo studies involving mastitis mouse models. In vitro results indicate that lactic acid was the primary substance inhibiting the E. coli pathogen. Meanwhile, treatment with L. plantarum KLDS 1.0344 can reduce cytokines’ mRNA expression levels in the inflammatory response of bMEC induced by LPS. In vivo, the use of this strain reduced the secretion of inflammatory factors IL-6, IL-1β, and TNF-α, and decreased the activity of myeloperoxidase (MPO), and inhibited the secretion of p-p65 and p-IκBα. These results indicate that L. plantarum KLDS 1.0344 pretreatment can reduce the expression of inflammatory factors by inhibiting the activation of NF-κB signaling pathway, thus exerting prevent the occurrence of inflammation in vivo. Our findings show that L. plantarum KLDS 1.0344 has excellent properties as an alternative to antibiotics and can be developed into lactic acid bacteria preparation to prevent mastitis disease.Qingxue ChenQingxue ChenSong WangSong WangJiayao GuoJiayao GuoQinggang XieSmith Etareri EvivieSmith Etareri EvivieYue SongBailiang LiBailiang LiGuicheng HuoGuicheng HuoFrontiers Media S.A.articleLactobacillus plantarummastitisantibacterialimmunomodulationsignal pathImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus plantarum mastitis antibacterial immunomodulation signal path Immunologic diseases. Allergy RC581-607
spellingShingle	Lactobacillus plantarum mastitis antibacterial immunomodulation signal path Immunologic diseases. Allergy RC581-607 Qingxue Chen Qingxue Chen Song Wang Song Wang Jiayao Guo Jiayao Guo Qinggang Xie Smith Etareri Evivie Smith Etareri Evivie Yue Song Bailiang Li Bailiang Li Guicheng Huo Guicheng Huo The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
description	Cow mastitis, which significantly lowers milk quality, is mainly caused by pathogenic bacteria such as E. coli. Previous studies have suggested that lactic acid bacteria can have antagonistic effects on pathogenic bacteria that cause mastitis. In the current study, we evaluated the in vitro and in vivo alleviative effects of L. plantarum KLDS 1.0344 in mastitis treatment. In vitro antibacterial experiments were performed using bovine mammary epithelial cell (bMEC), followed by in vivo studies involving mastitis mouse models. In vitro results indicate that lactic acid was the primary substance inhibiting the E. coli pathogen. Meanwhile, treatment with L. plantarum KLDS 1.0344 can reduce cytokines’ mRNA expression levels in the inflammatory response of bMEC induced by LPS. In vivo, the use of this strain reduced the secretion of inflammatory factors IL-6, IL-1β, and TNF-α, and decreased the activity of myeloperoxidase (MPO), and inhibited the secretion of p-p65 and p-IκBα. These results indicate that L. plantarum KLDS 1.0344 pretreatment can reduce the expression of inflammatory factors by inhibiting the activation of NF-κB signaling pathway, thus exerting prevent the occurrence of inflammation in vivo. Our findings show that L. plantarum KLDS 1.0344 has excellent properties as an alternative to antibiotics and can be developed into lactic acid bacteria preparation to prevent mastitis disease.
format	article
author	Qingxue Chen Qingxue Chen Song Wang Song Wang Jiayao Guo Jiayao Guo Qinggang Xie Smith Etareri Evivie Smith Etareri Evivie Yue Song Bailiang Li Bailiang Li Guicheng Huo Guicheng Huo
author_facet	Qingxue Chen Qingxue Chen Song Wang Song Wang Jiayao Guo Jiayao Guo Qinggang Xie Smith Etareri Evivie Smith Etareri Evivie Yue Song Bailiang Li Bailiang Li Guicheng Huo Guicheng Huo
author_sort	Qingxue Chen
title	The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
title_short	The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
title_full	The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
title_fullStr	The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
title_full_unstemmed	The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo
title_sort	protective effects of lactobacillus plantarum klds 1.0344 on lps-induced mastitis in vitro and in vivo
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1028dad9d1d946c282578545b2a36389
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The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo

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