A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis

Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase rchy1 in C. semilaevis (Cs-rchy1) was clon...

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Autores principales:	Yuxuan Sun, Ying Zhu, Peng Cheng, Mengqian Zhang, Na Wang, Zhongkai Cui, Min Wei, Wenteng Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cynoglossus semilaevis</i> <i>Cs-rchy1</i> ubiquitin ligases expression pattern RNA interference Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/1029e226c79d41a6936adca09820784e
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spelling	oai:doaj.org-article:1029e226c79d41a6936adca09820784e2021-11-25T16:20:00ZA Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>10.3390/ani111132652076-2615https://doaj.org/article/1029e226c79d41a6936adca09820784e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3265https://doaj.org/toc/2076-2615Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase <i>rchy1</i> in <i>C. semilaevis</i> (<i>Cs-rchy1</i>) was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5′UTR, 736 bp 3′UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. <i>Cs-rchy1</i> was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, <i>Cs-rchy1</i> could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that <i>Cs-rchy1</i> was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that <i>Cs-rchy1</i> knockdown resulted in the decline of <i>sox9</i> and <i>igf1</i> in ovarian cell line and down-regulation of <i>cyp19a</i> in the testicular cell line. These data suggested that <i>Cs-rchy1</i> might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis.Yuxuan SunYing ZhuPeng ChengMengqian ZhangNa WangZhongkai CuiMin WeiWenteng XuMDPI AGarticle<i>Cynoglossus semilaevis</i><i>Cs-rchy1</i>ubiquitin ligasesexpression patternRNA interferenceVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3265, p 3265 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cynoglossus semilaevis</i> <i>Cs-rchy1</i> ubiquitin ligases expression pattern RNA interference Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	<i>Cynoglossus semilaevis</i> <i>Cs-rchy1</i> ubiquitin ligases expression pattern RNA interference Veterinary medicine SF600-1100 Zoology QL1-991 Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
description	Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase <i>rchy1</i> in <i>C. semilaevis</i> (<i>Cs-rchy1</i>) was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5′UTR, 736 bp 3′UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. <i>Cs-rchy1</i> was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, <i>Cs-rchy1</i> could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that <i>Cs-rchy1</i> was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that <i>Cs-rchy1</i> knockdown resulted in the decline of <i>sox9</i> and <i>igf1</i> in ovarian cell line and down-regulation of <i>cyp19a</i> in the testicular cell line. These data suggested that <i>Cs-rchy1</i> might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis.
format	article
author	Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu
author_facet	Yuxuan Sun Ying Zhu Peng Cheng Mengqian Zhang Na Wang Zhongkai Cui Min Wei Wenteng Xu
author_sort	Yuxuan Sun
title	A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
title_short	A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
title_full	A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
title_fullStr	A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
title_full_unstemmed	A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>
title_sort	z-linked e3 ubiquitin ligase <i>cs-rchy1</i> is involved in gametogenesis in chinese tongue sole, <i>cynoglossus semilaevis</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1029e226c79d41a6936adca09820784e
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A Z-Linked E3 Ubiquitin Ligase <i>Cs-rchy1</i> Is Involved in Gametogenesis in Chinese Tongue Sole, <i>Cynoglossus semilaevis</i>

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