Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid.
The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses...
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oai:doaj.org-article:10330893d93146c7a9df0e59010bddda2021-11-18T09:01:57ZAberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid.1932-620310.1371/journal.pone.0070496https://doaj.org/article/10330893d93146c7a9df0e59010bddda2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936217/?tool=EBIhttps://doaj.org/toc/1932-6203The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP), the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i) Hsp90 was highly expressed in the skin of BP patients, whereas its serum levels were decreased and inversely associated with IgG autoantibody levels against the NC16A immunodominant region of the BP180 autoantigen, (ii) in contrast, neither aberrant levels of circulating Hsp90 nor any correlation of this protein with serum autoantibodies was found in a control cohort of autoimmune bullous disease patients with pemphigus vulgaris, (iii) Hsp90 was highly expressed in and restrictedly released from peripheral blood mononuclear cells of BP patients, and (iv) Hsp90 was potently induced in and restrictedly secreted from human keratinocyte (HaCaT) cells by BP serum and isolated anti-BP180 NC16A IgG autoantibodies, respectively. Our results reveal an upregulated Hsp90 expression at the site of inflammation and an autoantibody-mediated dysregulation of the intracellular and extracellular distribution of this chaperone in BP patients. These findings suggest that Hsp90 may play a pathophysiological role and represent a novel potential treatment target in BP.Stefan TukajKonrad KleszczyńskiKaterina VafiaStephanie GrothDamian MeyersburgPiotr TrzonkowskiRalf J LudwigDetlef ZillikensEnno SchmidtTobias W FischerMichael KasperkiewiczPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70496 (2013) |
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Medicine R Science Q Stefan Tukaj Konrad Kleszczyński Katerina Vafia Stephanie Groth Damian Meyersburg Piotr Trzonkowski Ralf J Ludwig Detlef Zillikens Enno Schmidt Tobias W Fischer Michael Kasperkiewicz Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| description |
The cell stress chaperone heat shock protein 90 (Hsp90) has been implicated in inflammatory responses and its inhibition has proven successful in different mouse models of autoimmune diseases, including epidermolysis bullosa acquisita. Here, we investigated expression levels and secretory responses of Hsp90 in patients with bullous pemphigoid (BP), the most common subepidermal autoimmune blistering skin disease. In comparison to healthy controls, the following observations were made: (i) Hsp90 was highly expressed in the skin of BP patients, whereas its serum levels were decreased and inversely associated with IgG autoantibody levels against the NC16A immunodominant region of the BP180 autoantigen, (ii) in contrast, neither aberrant levels of circulating Hsp90 nor any correlation of this protein with serum autoantibodies was found in a control cohort of autoimmune bullous disease patients with pemphigus vulgaris, (iii) Hsp90 was highly expressed in and restrictedly released from peripheral blood mononuclear cells of BP patients, and (iv) Hsp90 was potently induced in and restrictedly secreted from human keratinocyte (HaCaT) cells by BP serum and isolated anti-BP180 NC16A IgG autoantibodies, respectively. Our results reveal an upregulated Hsp90 expression at the site of inflammation and an autoantibody-mediated dysregulation of the intracellular and extracellular distribution of this chaperone in BP patients. These findings suggest that Hsp90 may play a pathophysiological role and represent a novel potential treatment target in BP. |
| format |
article |
| author |
Stefan Tukaj Konrad Kleszczyński Katerina Vafia Stephanie Groth Damian Meyersburg Piotr Trzonkowski Ralf J Ludwig Detlef Zillikens Enno Schmidt Tobias W Fischer Michael Kasperkiewicz |
| author_facet |
Stefan Tukaj Konrad Kleszczyński Katerina Vafia Stephanie Groth Damian Meyersburg Piotr Trzonkowski Ralf J Ludwig Detlef Zillikens Enno Schmidt Tobias W Fischer Michael Kasperkiewicz |
| author_sort |
Stefan Tukaj |
| title |
Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| title_short |
Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| title_full |
Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| title_fullStr |
Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| title_full_unstemmed |
Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| title_sort |
aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/10330893d93146c7a9df0e59010bddda |
| work_keys_str_mv |
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| _version_ |
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