Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production

Abstract Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting −/− mice were intratracheally sen...

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Autores principales:	Hiroki Nunokawa, Yusuke Murakami, Takashi Ishii, Tomoya Narita, Haruyuki Ishii, Hajime Takizawa, Naomi Yamashita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10335e65b5204b69a49af1b888466873
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spelling	oai:doaj.org-article:10335e65b5204b69a49af1b8884668732021-12-02T17:14:30ZCrucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production10.1038/s41598-021-92561-w2045-2322https://doaj.org/article/10335e65b5204b69a49af1b8884668732021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92561-whttps://doaj.org/toc/2045-2322Abstract Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting −/− mice were intratracheally sensitized with HDM, and the bronchoalveolar lavage fluid (BALF), sera, lungs, and mediastinal lymph nodes (MLNs) were analyzed. The total and HDM-specific serum IgE levels were lower in Sting −/− mice than in WT mice. B cell and IgE-positive B cell proportion in BALF and MLNs, respectively, was significantly lower in Sting −/− mice than in WT mice. Additionally, cyclic GMP-AMP, a STING ligand, augmented total and HDM-specific serum IgE levels and B cell proportion in BALF when applied in combination with HDM. To elucidate the role of STING in IgE production, follicular helper T (Tfh) cells, which are involved in B cell maturation, were investigated. Tfh cell proportion in MLNs decreased in Sting −/− mice, and IL-4 and IL-13 production by HDM-restimulated MLN cells from HDM-sensitized mice was decreased in Sting −/− mice compared with WT mice. Thus, STING plays an important role in the maturation and class switching of IgE-producing B cells in allergic inflammation via Tfh cells.Hiroki NunokawaYusuke MurakamiTakashi IshiiTomoya NaritaHaruyuki IshiiHajime TakizawaNaomi YamashitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroki Nunokawa Yusuke Murakami Takashi Ishii Tomoya Narita Haruyuki Ishii Hajime Takizawa Naomi Yamashita Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
description	Abstract Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting −/− mice were intratracheally sensitized with HDM, and the bronchoalveolar lavage fluid (BALF), sera, lungs, and mediastinal lymph nodes (MLNs) were analyzed. The total and HDM-specific serum IgE levels were lower in Sting −/− mice than in WT mice. B cell and IgE-positive B cell proportion in BALF and MLNs, respectively, was significantly lower in Sting −/− mice than in WT mice. Additionally, cyclic GMP-AMP, a STING ligand, augmented total and HDM-specific serum IgE levels and B cell proportion in BALF when applied in combination with HDM. To elucidate the role of STING in IgE production, follicular helper T (Tfh) cells, which are involved in B cell maturation, were investigated. Tfh cell proportion in MLNs decreased in Sting −/− mice, and IL-4 and IL-13 production by HDM-restimulated MLN cells from HDM-sensitized mice was decreased in Sting −/− mice compared with WT mice. Thus, STING plays an important role in the maturation and class switching of IgE-producing B cells in allergic inflammation via Tfh cells.
format	article
author	Hiroki Nunokawa Yusuke Murakami Takashi Ishii Tomoya Narita Haruyuki Ishii Hajime Takizawa Naomi Yamashita
author_facet	Hiroki Nunokawa Yusuke Murakami Takashi Ishii Tomoya Narita Haruyuki Ishii Hajime Takizawa Naomi Yamashita
author_sort	Hiroki Nunokawa
title	Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
title_short	Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
title_full	Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
title_fullStr	Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
title_full_unstemmed	Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
title_sort	crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced ige production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/10335e65b5204b69a49af1b888466873
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Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production

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