Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.

Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogen...

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Autores principales:	Feng Su, Xin Liu, Guanghui Liu, Yuan Yu, Yongsheng Wang, Yaping Jin, Guangdong Hu, Song Hua, Yong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1033d0667b6243f486e1641e98e73df0
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spelling	oai:doaj.org-article:1033d0667b6243f486e1641e98e73df02021-11-18T08:53:39ZEstablishment and evaluation of a stable cattle type II alveolar epithelial cell line.1932-620310.1371/journal.pone.0076036https://doaj.org/article/1033d0667b6243f486e1641e98e73df02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086682/?tool=EBIhttps://doaj.org/toc/1932-6203Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogenous immune research on bacterial invasion is necessary. In the present study, we first purified and immortalized type II AECs (AEC II cells) by transfecting them with a plasmid containing the human telomerase reverse trancriptase gene. We then tested whether or not the immortalized cells retained the basic physiological properties of primary AECs by reverse-transcription polymerase chain reaction and Western blot. Finally, we tested the secretion capacity of immortalized AEC II cells upon stimulation by bacterial invasion. The cattle type II alveolar epithelial cell line (HTERT-AEC II) that we established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. Thus, the cell line we established is a potential tool for research on the relationship between AECs and Mycobacterium tuberculosis.Feng SuXin LiuGuanghui LiuYuan YuYongsheng WangYaping JinGuangdong HuSong HuaYong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e76036 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Feng Su Xin Liu Guanghui Liu Yuan Yu Yongsheng Wang Yaping Jin Guangdong Hu Song Hua Yong Zhang Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
description	Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogenous immune research on bacterial invasion is necessary. In the present study, we first purified and immortalized type II AECs (AEC II cells) by transfecting them with a plasmid containing the human telomerase reverse trancriptase gene. We then tested whether or not the immortalized cells retained the basic physiological properties of primary AECs by reverse-transcription polymerase chain reaction and Western blot. Finally, we tested the secretion capacity of immortalized AEC II cells upon stimulation by bacterial invasion. The cattle type II alveolar epithelial cell line (HTERT-AEC II) that we established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. Thus, the cell line we established is a potential tool for research on the relationship between AECs and Mycobacterium tuberculosis.
format	article
author	Feng Su Xin Liu Guanghui Liu Yuan Yu Yongsheng Wang Yaping Jin Guangdong Hu Song Hua Yong Zhang
author_facet	Feng Su Xin Liu Guanghui Liu Yuan Yu Yongsheng Wang Yaping Jin Guangdong Hu Song Hua Yong Zhang
author_sort	Feng Su
title	Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
title_short	Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
title_full	Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
title_fullStr	Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
title_full_unstemmed	Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.
title_sort	establishment and evaluation of a stable cattle type ii alveolar epithelial cell line.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1033d0667b6243f486e1641e98e73df0
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Establishment and evaluation of a stable cattle type II alveolar epithelial cell line.

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