PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS

The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. A. Savchenko, E. V. Kasparov, S. S. Arutyunyan, A. G. Borisov, I. V. Kudryavtsev, A. V. Moshev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	b-lymphocytes chronic endometritis chronic adnexitis subpopulation structure antibacterial immunity low-affinity ige receptor Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/103fb3a169e44140a32afa066059f725
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:103fb3a169e44140a32afa066059f725
record_format	dspace
spelling	oai:doaj.org-article:103fb3a169e44140a32afa066059f7252021-11-18T08:03:45ZPHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS1563-06252313-741X10.15789/1563-0625-2016-4-379-384https://doaj.org/article/103fb3a169e44140a32afa066059f7252016-08-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1061https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders.A. A. SavchenkoE. V. KasparovS. S. ArutyunyanA. G. BorisovI. V. KudryavtsevA. V. MoshevSPb RAACIarticleb-lymphocyteschronic endometritischronic adnexitissubpopulation structureantibacterial immunitylow-affinity ige receptorImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 4, Pp 379-384 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	b-lymphocytes chronic endometritis chronic adnexitis subpopulation structure antibacterial immunity low-affinity ige receptor Immunologic diseases. Allergy RC581-607
spellingShingle	b-lymphocytes chronic endometritis chronic adnexitis subpopulation structure antibacterial immunity low-affinity ige receptor Immunologic diseases. Allergy RC581-607 A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
description	The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders.
format	article
author	A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev
author_facet	A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev
author_sort	A. A. Savchenko
title	PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
title_short	PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
title_full	PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
title_fullStr	PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
title_full_unstemmed	PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
title_sort	phenotypic profile of b-lymphocytes in women with chronic endometritis and adnexitis
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/103fb3a169e44140a32afa066059f725
work_keys_str_mv	AT aasavchenko phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis AT evkasparov phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis AT ssarutyunyan phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis AT agborisov phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis AT ivkudryavtsev phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis AT avmoshev phenotypicprofileofblymphocytesinwomenwithchronicendometritisandadnexitis
_version_	1718422396765995008

PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS

Ejemplares similares