Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6

The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its cofactor Cdc6 and also report an in vitro reconstitu...

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Auteurs principaux:	Jan Marten Schmidt, Franziska Bleichert
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2020
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/104772f99fe64bef9c4c622c3fb6069d
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id	oai:doaj.org-article:104772f99fe64bef9c4c622c3fb6069d
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spelling	oai:doaj.org-article:104772f99fe64bef9c4c622c3fb6069d2021-12-02T19:02:25ZStructural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc610.1038/s41467-020-18067-72041-1723https://doaj.org/article/104772f99fe64bef9c4c622c3fb6069d2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18067-7https://doaj.org/toc/2041-1723The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its cofactor Cdc6 and also report an in vitro reconstitution system for Drosophila Mcm2–7 loading, revealing unexpected features of ORC’s DNA binding and remodeling mechanism during Mcm2–7 loading.Jan Marten SchmidtFranziska BleichertNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jan Marten Schmidt Franziska Bleichert Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
description	The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its cofactor Cdc6 and also report an in vitro reconstitution system for Drosophila Mcm2–7 loading, revealing unexpected features of ORC’s DNA binding and remodeling mechanism during Mcm2–7 loading.
format	article
author	Jan Marten Schmidt Franziska Bleichert
author_facet	Jan Marten Schmidt Franziska Bleichert
author_sort	Jan Marten Schmidt
title	Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
title_short	Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
title_full	Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
title_fullStr	Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
title_full_unstemmed	Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
title_sort	structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader cdc6
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/104772f99fe64bef9c4c622c3fb6069d
work_keys_str_mv	AT janmartenschmidt structuralmechanismforreplicationoriginbindingandremodelingbyametazoanoriginrecognitioncomplexanditscoloadercdc6 AT franziskableichert structuralmechanismforreplicationoriginbindingandremodelingbyametazoanoriginrecognitioncomplexanditscoloadercdc6
_version_	1718377249404616704

Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6

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