Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds

The aim of the current research was to analyze the population structure and expression of KISS1 gene associated with reproductive traits through Follicle Stimulating Hormone (FSH) level and DNA sequencing analysis based on KISS1 gene. Genotypes of 23 goat does consist of Kacang goats (n=7), Kejobong...

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Autores principales:	A. Febriana, S. Sutopo, E. Kurnianto, W. Widiyanto
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University, Faculty of Animal and Agricultural Sciences 2021
Materias:	fsh haplotype indonesian native goat kiss1 gene phylogenetic tree Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/10496e43b6454c559e50a80cc2556c06
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spelling	oai:doaj.org-article:10496e43b6454c559e50a80cc2556c062021-12-02T20:46:58ZPhylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds2087-82732460-627810.14710/jitaa.46.4.282-294https://doaj.org/article/10496e43b6454c559e50a80cc2556c062021-12-01T00:00:00Zhttps://ejournal.undip.ac.id/index.php/jitaa/article/view/41168https://doaj.org/toc/2087-8273https://doaj.org/toc/2460-6278The aim of the current research was to analyze the population structure and expression of KISS1 gene associated with reproductive traits through Follicle Stimulating Hormone (FSH) level and DNA sequencing analysis based on KISS1 gene. Genotypes of 23 goat does consist of Kacang goats (n=7), Kejobong goats (n=8) and Senduro goats (n=8) were investigated using DNA sequencing, 16 out of 23 samples were synchronized to examine their FSH level using ELISA method. The data were analyzed using MEGA X software for DNA sequences and General Linier Model (GLM) for FSH plasma level. The phylogenetic tree showed the high homology between Indonesian native goats with other species showing a gene conservatism. A significantly higher FSH plasma levels were obtained from Kacang and Kejobong than Senduro goat (P = 0.002), litter size (LS) 3 than LS 1 (P = 0.0175), further CAATGCGCAACGCT haplotype and GA genotype at g.2459 G>A have a higher FSH plasma than other haplotypes and genotypes (P = 0.0027; P<0.0001) and are associated with high LS (3.0±0.18). Neither sample collection times nor parities have different significantly. The current trial indicated that CAATGCGCAACGCT haplotype and GA genotype at g.2459 G>A were correlated with reproductive traits.A. FebrianaS. SutopoE. KurniantoW. WidiyantoDiponegoro University, Faculty of Animal and Agricultural Sciencesarticlefshhaplotypeindonesian native goatkiss1 genephylogenetic treeAnimal cultureSF1-1100Veterinary medicineSF600-1100ENJournal of the Indonesian Tropical Animal Agriculture, Vol 46, Iss 4, Pp 282-294 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fsh haplotype indonesian native goat kiss1 gene phylogenetic tree Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	fsh haplotype indonesian native goat kiss1 gene phylogenetic tree Animal culture SF1-1100 Veterinary medicine SF600-1100 A. Febriana S. Sutopo E. Kurnianto W. Widiyanto Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
description	The aim of the current research was to analyze the population structure and expression of KISS1 gene associated with reproductive traits through Follicle Stimulating Hormone (FSH) level and DNA sequencing analysis based on KISS1 gene. Genotypes of 23 goat does consist of Kacang goats (n=7), Kejobong goats (n=8) and Senduro goats (n=8) were investigated using DNA sequencing, 16 out of 23 samples were synchronized to examine their FSH level using ELISA method. The data were analyzed using MEGA X software for DNA sequences and General Linier Model (GLM) for FSH plasma level. The phylogenetic tree showed the high homology between Indonesian native goats with other species showing a gene conservatism. A significantly higher FSH plasma levels were obtained from Kacang and Kejobong than Senduro goat (P = 0.002), litter size (LS) 3 than LS 1 (P = 0.0175), further CAATGCGCAACGCT haplotype and GA genotype at g.2459 G>A have a higher FSH plasma than other haplotypes and genotypes (P = 0.0027; P<0.0001) and are associated with high LS (3.0±0.18). Neither sample collection times nor parities have different significantly. The current trial indicated that CAATGCGCAACGCT haplotype and GA genotype at g.2459 G>A were correlated with reproductive traits.
format	article
author	A. Febriana S. Sutopo E. Kurnianto W. Widiyanto
author_facet	A. Febriana S. Sutopo E. Kurnianto W. Widiyanto
author_sort	A. Febriana
title	Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
title_short	Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
title_full	Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
title_fullStr	Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
title_full_unstemmed	Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds
title_sort	phylogenetic study and association between prominent genotype and haplotype of kiss1 gene with fsh level in idonesian native goat breeds
publisher	Diponegoro University, Faculty of Animal and Agricultural Sciences
publishDate	2021
url	https://doaj.org/article/10496e43b6454c559e50a80cc2556c06
work_keys_str_mv	AT afebriana phylogeneticstudyandassociationbetweenprominentgenotypeandhaplotypeofkiss1genewithfshlevelinidonesiannativegoatbreeds AT ssutopo phylogeneticstudyandassociationbetweenprominentgenotypeandhaplotypeofkiss1genewithfshlevelinidonesiannativegoatbreeds AT ekurnianto phylogeneticstudyandassociationbetweenprominentgenotypeandhaplotypeofkiss1genewithfshlevelinidonesiannativegoatbreeds AT wwidiyanto phylogeneticstudyandassociationbetweenprominentgenotypeandhaplotypeofkiss1genewithfshlevelinidonesiannativegoatbreeds
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Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH level in Idonesian native goat breeds

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