Detection of dietetically absorbed maize-derived microRNAs in pigs

Abstract MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-der...

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Autores principales:	Yi Luo, Pengjun Wang, Xun Wang, Yuhao Wang, Zhiping Mu, Qingzhi Li, Yuhua Fu, Juan Xiao, Guojun Li, Yao Ma, Yiren Gu, Long Jin, Jideng Ma, Qianzi Tang, Anan Jiang, Xuewei Li, Mingzhou Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/104a265438d94f888802fceb14144909
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spelling	oai:doaj.org-article:104a265438d94f888802fceb141449092021-12-02T15:05:43ZDetection of dietetically absorbed maize-derived microRNAs in pigs10.1038/s41598-017-00488-y2045-2322https://doaj.org/article/104a265438d94f888802fceb141449092017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00488-yhttps://doaj.org/toc/2045-2322Abstract MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plant miRNAs could regulate mammalian gene expression, and these studies have broadened our view of cross-kingdom communication. In the present study, we evaluated miRNA levels in cooked maize-containing chow diets, and found that plant miRNAs were resistant to the harsh cooking conditions to a certain extent. After feeding fresh maize to pigs (7 days), maize-derived miRNAs could be detected in porcine tissues and serum, and the authenticity of these plant miRNAs was confirmed by using oxidization reactions. Furthermore, in vivo and in vitro experiments demonstrated that dietary maize miRNAs could cross the gastrointestinal tract and enter the porcine bloodstream. In the porcine cells, we found that plant miRNAs are very likely to specifically target their endogenous porcine mRNAs and influence gene expression in a fashion similar to that of mammalian miRNAs. Our results indicate that maize-derived miRNAs can cross the gastrointestinal tract and present in pigs, and these exogenous miRNAs have the potential to regulate mammalian gene expression.Yi LuoPengjun WangXun WangYuhao WangZhiping MuQingzhi LiYuhua FuJuan XiaoGuojun LiYao MaYiren GuLong JinJideng MaQianzi TangAnan JiangXuewei LiMingzhou LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Luo Pengjun Wang Xun Wang Yuhao Wang Zhiping Mu Qingzhi Li Yuhua Fu Juan Xiao Guojun Li Yao Ma Yiren Gu Long Jin Jideng Ma Qianzi Tang Anan Jiang Xuewei Li Mingzhou Li Detection of dietetically absorbed maize-derived microRNAs in pigs
description	Abstract MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plant miRNAs could regulate mammalian gene expression, and these studies have broadened our view of cross-kingdom communication. In the present study, we evaluated miRNA levels in cooked maize-containing chow diets, and found that plant miRNAs were resistant to the harsh cooking conditions to a certain extent. After feeding fresh maize to pigs (7 days), maize-derived miRNAs could be detected in porcine tissues and serum, and the authenticity of these plant miRNAs was confirmed by using oxidization reactions. Furthermore, in vivo and in vitro experiments demonstrated that dietary maize miRNAs could cross the gastrointestinal tract and enter the porcine bloodstream. In the porcine cells, we found that plant miRNAs are very likely to specifically target their endogenous porcine mRNAs and influence gene expression in a fashion similar to that of mammalian miRNAs. Our results indicate that maize-derived miRNAs can cross the gastrointestinal tract and present in pigs, and these exogenous miRNAs have the potential to regulate mammalian gene expression.
format	article
author	Yi Luo Pengjun Wang Xun Wang Yuhao Wang Zhiping Mu Qingzhi Li Yuhua Fu Juan Xiao Guojun Li Yao Ma Yiren Gu Long Jin Jideng Ma Qianzi Tang Anan Jiang Xuewei Li Mingzhou Li
author_facet	Yi Luo Pengjun Wang Xun Wang Yuhao Wang Zhiping Mu Qingzhi Li Yuhua Fu Juan Xiao Guojun Li Yao Ma Yiren Gu Long Jin Jideng Ma Qianzi Tang Anan Jiang Xuewei Li Mingzhou Li
author_sort	Yi Luo
title	Detection of dietetically absorbed maize-derived microRNAs in pigs
title_short	Detection of dietetically absorbed maize-derived microRNAs in pigs
title_full	Detection of dietetically absorbed maize-derived microRNAs in pigs
title_fullStr	Detection of dietetically absorbed maize-derived microRNAs in pigs
title_full_unstemmed	Detection of dietetically absorbed maize-derived microRNAs in pigs
title_sort	detection of dietetically absorbed maize-derived micrornas in pigs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/104a265438d94f888802fceb14144909
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Detection of dietetically absorbed maize-derived microRNAs in pigs

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