Bioinformatic and expression analysis of the Brassica napus L. cyclophilins

Abstract Cyclophilins (CYPs) are a group of ubiquitous proteins characterized by their ability to bind to the immunosuppressive drug cyclosporin A. The CYP family occurs in a wide range of organisms and contains a conserved peptidyl-prolyl cis/trans isomerase domain. In addition to fulfilling a basi...

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Autores principales:	Patrizia Hanhart, Melanie Thieß, Khalid Amari, Krzysztof Bajdzienko, Patrick Giavalisco, Manfred Heinlein, Julia Kehr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/104ad6a317d944bf814a34765805b572
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spelling	oai:doaj.org-article:104ad6a317d944bf814a34765805b5722021-12-02T11:53:01ZBioinformatic and expression analysis of the Brassica napus L. cyclophilins10.1038/s41598-017-01596-52045-2322https://doaj.org/article/104ad6a317d944bf814a34765805b5722017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01596-5https://doaj.org/toc/2045-2322Abstract Cyclophilins (CYPs) are a group of ubiquitous proteins characterized by their ability to bind to the immunosuppressive drug cyclosporin A. The CYP family occurs in a wide range of organisms and contains a conserved peptidyl-prolyl cis/trans isomerase domain. In addition to fulfilling a basic role in protein folding, CYPs may also play diverse important roles, e.g. in protein degradation, mRNA processing, development, and stress responses. We performed a genome-wide database survey and identified a total of 94 CYP genes encoding 91 distinct proteins. Sequence alignment analysis of the putative BnCYP cyclophilin-like domains revealed highly conserved motifs. By using RNA-Seq, we could verify the presence of 77 BnCYP genes under control conditions. To identify phloem-specific BnCYP proteins in a complementary approach, we used LC-MS/MS to determine protein abundances in leaf and phloem extracts. We detected 26 BnCYPs in total with 12 being unique to phloem sap. Our analysis provides the basis for future studies concentrating on the functional characterization of individual members of this gene family in a plant of dual importance: as a crop and a model system for polyploidization and long-distance signalling.Patrizia HanhartMelanie ThießKhalid AmariKrzysztof BajdzienkoPatrick GiavaliscoManfred HeinleinJulia KehrNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrizia Hanhart Melanie Thieß Khalid Amari Krzysztof Bajdzienko Patrick Giavalisco Manfred Heinlein Julia Kehr Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
description	Abstract Cyclophilins (CYPs) are a group of ubiquitous proteins characterized by their ability to bind to the immunosuppressive drug cyclosporin A. The CYP family occurs in a wide range of organisms and contains a conserved peptidyl-prolyl cis/trans isomerase domain. In addition to fulfilling a basic role in protein folding, CYPs may also play diverse important roles, e.g. in protein degradation, mRNA processing, development, and stress responses. We performed a genome-wide database survey and identified a total of 94 CYP genes encoding 91 distinct proteins. Sequence alignment analysis of the putative BnCYP cyclophilin-like domains revealed highly conserved motifs. By using RNA-Seq, we could verify the presence of 77 BnCYP genes under control conditions. To identify phloem-specific BnCYP proteins in a complementary approach, we used LC-MS/MS to determine protein abundances in leaf and phloem extracts. We detected 26 BnCYPs in total with 12 being unique to phloem sap. Our analysis provides the basis for future studies concentrating on the functional characterization of individual members of this gene family in a plant of dual importance: as a crop and a model system for polyploidization and long-distance signalling.
format	article
author	Patrizia Hanhart Melanie Thieß Khalid Amari Krzysztof Bajdzienko Patrick Giavalisco Manfred Heinlein Julia Kehr
author_facet	Patrizia Hanhart Melanie Thieß Khalid Amari Krzysztof Bajdzienko Patrick Giavalisco Manfred Heinlein Julia Kehr
author_sort	Patrizia Hanhart
title	Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
title_short	Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
title_full	Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
title_fullStr	Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
title_full_unstemmed	Bioinformatic and expression analysis of the Brassica napus L. cyclophilins
title_sort	bioinformatic and expression analysis of the brassica napus l. cyclophilins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/104ad6a317d944bf814a34765805b572
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Bioinformatic and expression analysis of the Brassica napus L. cyclophilins

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