Versatile protein tagging in cells with split fluorescent protein

Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introducti...

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Autores principales:	Daichi Kamiyama, Sayaka Sekine, Benjamin Barsi-Rhyne, Jeffrey Hu, Baohui Chen, Luke A. Gilbert, Hiroaki Ishikawa, Manuel D. Leonetti, Wallace F. Marshall, Jonathan S. Weissman, Bo Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1050b0556bdb46ffae8a8e6c60a400b8
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spelling	oai:doaj.org-article:1050b0556bdb46ffae8a8e6c60a400b82021-12-02T15:34:31ZVersatile protein tagging in cells with split fluorescent protein10.1038/ncomms110462041-1723https://doaj.org/article/1050b0556bdb46ffae8a8e6c60a400b82016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms11046https://doaj.org/toc/2041-1723Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introduction into genomic loci using CRISPR/Cas9.Daichi KamiyamaSayaka SekineBenjamin Barsi-RhyneJeffrey HuBaohui ChenLuke A. GilbertHiroaki IshikawaManuel D. LeonettiWallace F. MarshallJonathan S. WeissmanBo HuangNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Daichi Kamiyama Sayaka Sekine Benjamin Barsi-Rhyne Jeffrey Hu Baohui Chen Luke A. Gilbert Hiroaki Ishikawa Manuel D. Leonetti Wallace F. Marshall Jonathan S. Weissman Bo Huang Versatile protein tagging in cells with split fluorescent protein
description	Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introduction into genomic loci using CRISPR/Cas9.
format	article
author	Daichi Kamiyama Sayaka Sekine Benjamin Barsi-Rhyne Jeffrey Hu Baohui Chen Luke A. Gilbert Hiroaki Ishikawa Manuel D. Leonetti Wallace F. Marshall Jonathan S. Weissman Bo Huang
author_facet	Daichi Kamiyama Sayaka Sekine Benjamin Barsi-Rhyne Jeffrey Hu Baohui Chen Luke A. Gilbert Hiroaki Ishikawa Manuel D. Leonetti Wallace F. Marshall Jonathan S. Weissman Bo Huang
author_sort	Daichi Kamiyama
title	Versatile protein tagging in cells with split fluorescent protein
title_short	Versatile protein tagging in cells with split fluorescent protein
title_full	Versatile protein tagging in cells with split fluorescent protein
title_fullStr	Versatile protein tagging in cells with split fluorescent protein
title_full_unstemmed	Versatile protein tagging in cells with split fluorescent protein
title_sort	versatile protein tagging in cells with split fluorescent protein
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/1050b0556bdb46ffae8a8e6c60a400b8
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Versatile protein tagging in cells with split fluorescent protein

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