Versatile protein tagging in cells with split fluorescent protein
Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introducti...
Guardado en:
Autores principales: | Daichi Kamiyama, Sayaka Sekine, Benjamin Barsi-Rhyne, Jeffrey Hu, Baohui Chen, Luke A. Gilbert, Hiroaki Ishikawa, Manuel D. Leonetti, Wallace F. Marshall, Jonathan S. Weissman, Bo Huang |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2016
|
Materias: | |
Acceso en línea: | https://doaj.org/article/1050b0556bdb46ffae8a8e6c60a400b8 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Multiplexed labeling of cellular proteins with split fluorescent protein tags
por: Ryo Tamura, et al.
Publicado: (2021) -
Improved split fluorescent proteins for endogenous protein labeling
por: Siyu Feng, et al.
Publicado: (2017) -
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing
por: Olena S. Oliinyk, et al.
Publicado: (2019) -
Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2
por: Hyerim Park, et al.
Publicado: (2017) -
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.
por: Takuya Kobayashi, et al.
Publicado: (2008)