Versatile protein tagging in cells with split fluorescent protein
Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introducti...
Saved in:
Main Authors: | Daichi Kamiyama, Sayaka Sekine, Benjamin Barsi-Rhyne, Jeffrey Hu, Baohui Chen, Luke A. Gilbert, Hiroaki Ishikawa, Manuel D. Leonetti, Wallace F. Marshall, Jonathan S. Weissman, Bo Huang |
---|---|
Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2016
|
Subjects: | |
Online Access: | https://doaj.org/article/1050b0556bdb46ffae8a8e6c60a400b8 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Multiplexed labeling of cellular proteins with split fluorescent protein tags
by: Ryo Tamura, et al.
Published: (2021) -
Improved split fluorescent proteins for endogenous protein labeling
by: Siyu Feng, et al.
Published: (2017) -
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing
by: Olena S. Oliinyk, et al.
Published: (2019) -
Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2
by: Hyerim Park, et al.
Published: (2017) -
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.
by: Takuya Kobayashi, et al.
Published: (2008)