Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens

Abstract Females of social Hymenoptera only mate at the beginning of their adult lives and produce offspring until their death. In most ant species, queens live for over a decade, indicating that ant queens can store large numbers of spermatozoa throughout their long lives. To reveal the prolonged s...

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Autores principales:	Ayako Gotoh, Shuji Shigenobu, Katsushi Yamaguchi, Satoru Kobayashi, Fuminori Ito, Kazuki Tsuji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10537c13525341b691aa2a9af452e421
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spelling	oai:doaj.org-article:10537c13525341b691aa2a9af452e4212021-12-02T11:53:13ZTranscriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens10.1038/s41598-017-05818-82045-2322https://doaj.org/article/10537c13525341b691aa2a9af452e4212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05818-8https://doaj.org/toc/2045-2322Abstract Females of social Hymenoptera only mate at the beginning of their adult lives and produce offspring until their death. In most ant species, queens live for over a decade, indicating that ant queens can store large numbers of spermatozoa throughout their long lives. To reveal the prolonged sperm storage mechanisms, we identified enriched genes in the sperm-storage organ (spermatheca) relative to those in body samples in Crematogaster osakensis queens using the RNA-sequencing method. The genes encoding antioxidant enzymes, proteases, and extracellular matrix-related genes, and novel genes that have no similar sequences in the public databases were identified. We also performed differential expression analyses between the virgin and mated spermathecae or between the spermathecae at 1-week and 1-year after mating, to identify genes altered by the mating status or by the sperm storage period, respectively. Gene Ontology enrichment analyses suggested that antioxidant function is enhanced in the spermatheca at 1-week after mating compared with the virgin spermatheca and the spermatheca at 1-year after mating. In situ hybridization analyses of 128 selected contigs revealed that 12 contigs were particular to the spermatheca. These genes have never been reported in the reproductive organs of insect females, suggesting specialized roles in ant spermatheca.Ayako GotohShuji ShigenobuKatsushi YamaguchiSatoru KobayashiFuminori ItoKazuki TsujiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayako Gotoh Shuji Shigenobu Katsushi Yamaguchi Satoru Kobayashi Fuminori Ito Kazuki Tsuji Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
description	Abstract Females of social Hymenoptera only mate at the beginning of their adult lives and produce offspring until their death. In most ant species, queens live for over a decade, indicating that ant queens can store large numbers of spermatozoa throughout their long lives. To reveal the prolonged sperm storage mechanisms, we identified enriched genes in the sperm-storage organ (spermatheca) relative to those in body samples in Crematogaster osakensis queens using the RNA-sequencing method. The genes encoding antioxidant enzymes, proteases, and extracellular matrix-related genes, and novel genes that have no similar sequences in the public databases were identified. We also performed differential expression analyses between the virgin and mated spermathecae or between the spermathecae at 1-week and 1-year after mating, to identify genes altered by the mating status or by the sperm storage period, respectively. Gene Ontology enrichment analyses suggested that antioxidant function is enhanced in the spermatheca at 1-week after mating compared with the virgin spermatheca and the spermatheca at 1-year after mating. In situ hybridization analyses of 128 selected contigs revealed that 12 contigs were particular to the spermatheca. These genes have never been reported in the reproductive organs of insect females, suggesting specialized roles in ant spermatheca.
format	article
author	Ayako Gotoh Shuji Shigenobu Katsushi Yamaguchi Satoru Kobayashi Fuminori Ito Kazuki Tsuji
author_facet	Ayako Gotoh Shuji Shigenobu Katsushi Yamaguchi Satoru Kobayashi Fuminori Ito Kazuki Tsuji
author_sort	Ayako Gotoh
title	Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
title_short	Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
title_full	Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
title_fullStr	Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
title_full_unstemmed	Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
title_sort	transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/10537c13525341b691aa2a9af452e421
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Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens

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