Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip

Zearalenone (ZEN) is a mycotoxin produced by fungal species which are widespread in nature and commonly contaminate many cereal grains, such as wheat, maize, corn, barley, and other cereal grains. An immunochromatographic (ICA) test strip was developed for the rapid simultaneous detection of ZEN and...

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Autores principales:	Kai Hao, Steven Suryoprabowo, Shanshan Song, Liqiang Liu, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunochromatographic assay monoclonal antibody mycotoxin strip test zearalenone Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1058039fa60e42ae8e2837d9080d8e6a
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spelling	oai:doaj.org-article:1058039fa60e42ae8e2837d9080d8e6a2021-11-26T11:19:46ZRapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip0954-01051465-344310.1080/09540105.2017.1406461https://doaj.org/article/1058039fa60e42ae8e2837d9080d8e6a2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1406461https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Zearalenone (ZEN) is a mycotoxin produced by fungal species which are widespread in nature and commonly contaminate many cereal grains, such as wheat, maize, corn, barley, and other cereal grains. An immunochromatographic (ICA) test strip was developed for the rapid simultaneous detection of ZEN and its metabolite in corn flour samples. For the ICA test, antigen (ZEN-BSA) and goat anti-mouse IgG were, respectively, drawn on nitrocellulose membrane as control line and test line. Under the optimized condition, the cut-off limits of test strips for ZEN, α-ZAL, and β-ZAL were found to be 0.5 ng/mL in 0.01 M PBS and 50 ng/mL in corn flour samples, meanwhile α-ZOL, β-ZOL, and ZEA were found to be 1 ng/mL in 0.01 M PBS and 75 ng/mL in corn flour samples. Our data indicate that the ICA is sensitive, rapid, cost-effective, and specific on-site screening for ZEN and its metabolite.Kai HaoSteven SuryoprabowoShanshan SongLiqiang LiuHua KuangTaylor & Francis Grouparticleimmunochromatographic assaymonoclonal antibodymycotoxinstrip testzearalenoneAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 498-510 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunochromatographic assay monoclonal antibody mycotoxin strip test zearalenone Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunochromatographic assay monoclonal antibody mycotoxin strip test zearalenone Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
description	Zearalenone (ZEN) is a mycotoxin produced by fungal species which are widespread in nature and commonly contaminate many cereal grains, such as wheat, maize, corn, barley, and other cereal grains. An immunochromatographic (ICA) test strip was developed for the rapid simultaneous detection of ZEN and its metabolite in corn flour samples. For the ICA test, antigen (ZEN-BSA) and goat anti-mouse IgG were, respectively, drawn on nitrocellulose membrane as control line and test line. Under the optimized condition, the cut-off limits of test strips for ZEN, α-ZAL, and β-ZAL were found to be 0.5 ng/mL in 0.01 M PBS and 50 ng/mL in corn flour samples, meanwhile α-ZOL, β-ZOL, and ZEA were found to be 1 ng/mL in 0.01 M PBS and 75 ng/mL in corn flour samples. Our data indicate that the ICA is sensitive, rapid, cost-effective, and specific on-site screening for ZEN and its metabolite.
format	article
author	Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang
author_facet	Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang
author_sort	Kai Hao
title	Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
title_short	Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
title_full	Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
title_fullStr	Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
title_full_unstemmed	Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
title_sort	rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/1058039fa60e42ae8e2837d9080d8e6a
work_keys_str_mv	AT kaihao rapiddetectionofzearalenoneanditsmetaboliteincornflourwiththeimmunochromatographicteststrip AT stevensuryoprabowo rapiddetectionofzearalenoneanditsmetaboliteincornflourwiththeimmunochromatographicteststrip AT shanshansong rapiddetectionofzearalenoneanditsmetaboliteincornflourwiththeimmunochromatographicteststrip AT liqiangliu rapiddetectionofzearalenoneanditsmetaboliteincornflourwiththeimmunochromatographicteststrip AT huakuang rapiddetectionofzearalenoneanditsmetaboliteincornflourwiththeimmunochromatographicteststrip
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Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip

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