Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The developm...

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Autores principales:	K V Ravishankar, R Vasudeva, B Hemanth, P Nischita, B R Sthapit, V A Parthasarathy, V R Rao
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2021
Materias:	cross-species amplification garcinia indica microsatellite markers next-generation sequencing (ngs) Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/10580a4c300540cb98e2750a9f6e64fa
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spelling	oai:doaj.org-article:10580a4c300540cb98e2750a9f6e64fa2021-12-02T19:45:41ZIsolation and characterization of microsatellite markers from Garcinia indica and cross species amplification0973-354X2582-489910.24154/JHS.2021.v16i01.014https://doaj.org/article/10580a4c300540cb98e2750a9f6e64fa2021-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/1128https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)K V RavishankarR VasudevaB HemanthP NischitaB R SthapitV A ParthasarathyV R RaoSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlecross-species amplificationgarcinia indicamicrosatellite markersnext-generation sequencing (ngs)Plant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 16, Iss 1, Pp 125-129 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cross-species amplification garcinia indica microsatellite markers next-generation sequencing (ngs) Plant culture SB1-1110
spellingShingle	cross-species amplification garcinia indica microsatellite markers next-generation sequencing (ngs) Plant culture SB1-1110 K V Ravishankar R Vasudeva B Hemanth P Nischita B R Sthapit V A Parthasarathy V R Rao Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
description	Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)
format	article
author	K V Ravishankar R Vasudeva B Hemanth P Nischita B R Sthapit V A Parthasarathy V R Rao
author_facet	K V Ravishankar R Vasudeva B Hemanth P Nischita B R Sthapit V A Parthasarathy V R Rao
author_sort	K V Ravishankar
title	Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
title_short	Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
title_full	Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
title_fullStr	Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
title_full_unstemmed	Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification
title_sort	isolation and characterization of microsatellite markers from garcinia indica and cross species amplification
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2021
url	https://doaj.org/article/10580a4c300540cb98e2750a9f6e64fa
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Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

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