Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil

Salt stress is one of the important adverse conditions affecting bacterium growth. How bacteria isolated from greenhouse soil cope with salt stress and regulate the genes responsible for salt tolerance are still unclear. We conducted RNA transcriptome profiling of genes contributing to the salt tole...

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Autores principales:	Zhang Jian, Xiao Qingqing, Guo Tingting, Wang Pengcheng
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	bacteria chemicals gene expression facility soil salt resistance Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/105e365a3c8b4249b6d2067c6eacb856
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spelling	oai:doaj.org-article:105e365a3c8b4249b6d2067c6eacb8562021-12-05T14:10:43ZEffect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil2391-542010.1515/chem-2020-0181https://doaj.org/article/105e365a3c8b4249b6d2067c6eacb8562021-01-01T00:00:00Zhttps://doi.org/10.1515/chem-2020-0181https://doaj.org/toc/2391-5420Salt stress is one of the important adverse conditions affecting bacterium growth. How bacteria isolated from greenhouse soil cope with salt stress and regulate the genes responsible for salt tolerance are still unclear. We conducted RNA transcriptome profiling of genes contributing to the salt tolerance of a Bacillus sp. strain (“SX4”) obtained from salinized soil. Results showed that NaCl effectively regulated the growth of “SX4” in terms of cell length and colony-forming unit number decrease. A total of 121 upregulated and 346 downregulated genes were detected under salt stress with reference to the control. The largest numbers of differential expression genes were 17 in carbon metabolism, 13 in the biosynthesis of amino acids, 10 in a two-component system, and 10 in ABC transporter pathways for adapting to salt stress. Our data revealed that cation, electron and transmembrane transport, and catalytic activity play important roles in the resistance of bacterial cells to salt ions. Single-nucleotide polymorphism and the mutation of base pair T:A to C:G play potential roles in the adaptation of “SX4” to high NaCl concentrations. The findings from this study provide new insights into the molecular mechanisms of strain “SX4” and will be helpful in promoting the application of salt-tolerant bacteria.Zhang JianXiao QingqingGuo TingtingWang PengchengDe Gruyterarticlebacteriachemicalsgene expressionfacility soilsalt resistanceChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 9-22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria chemicals gene expression facility soil salt resistance Chemistry QD1-999
spellingShingle	bacteria chemicals gene expression facility soil salt resistance Chemistry QD1-999 Zhang Jian Xiao Qingqing Guo Tingting Wang Pengcheng Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
description	Salt stress is one of the important adverse conditions affecting bacterium growth. How bacteria isolated from greenhouse soil cope with salt stress and regulate the genes responsible for salt tolerance are still unclear. We conducted RNA transcriptome profiling of genes contributing to the salt tolerance of a Bacillus sp. strain (“SX4”) obtained from salinized soil. Results showed that NaCl effectively regulated the growth of “SX4” in terms of cell length and colony-forming unit number decrease. A total of 121 upregulated and 346 downregulated genes were detected under salt stress with reference to the control. The largest numbers of differential expression genes were 17 in carbon metabolism, 13 in the biosynthesis of amino acids, 10 in a two-component system, and 10 in ABC transporter pathways for adapting to salt stress. Our data revealed that cation, electron and transmembrane transport, and catalytic activity play important roles in the resistance of bacterial cells to salt ions. Single-nucleotide polymorphism and the mutation of base pair T:A to C:G play potential roles in the adaptation of “SX4” to high NaCl concentrations. The findings from this study provide new insights into the molecular mechanisms of strain “SX4” and will be helpful in promoting the application of salt-tolerant bacteria.
format	article
author	Zhang Jian Xiao Qingqing Guo Tingting Wang Pengcheng
author_facet	Zhang Jian Xiao Qingqing Guo Tingting Wang Pengcheng
author_sort	Zhang Jian
title	Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
title_short	Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
title_full	Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
title_fullStr	Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
title_full_unstemmed	Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil
title_sort	effect of sodium chloride on the expression of genes involved in the salt tolerance of bacillus sp. strain “sx4” isolated from salinized greenhouse soil
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/105e365a3c8b4249b6d2067c6eacb856
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Effect of sodium chloride on the expression of genes involved in the salt tolerance of Bacillus sp. strain “SX4” isolated from salinized greenhouse soil

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