Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery

ABSTRACT Perturbation of vaginal microbiome of reproductive-age women influences all the phases of a woman's reproductive life. Although studies have shown that dynamic changes in vaginal microbiome can affect pregnancy, its role in secondary infertility (i.e., inability to become pregnant or t...

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Autores principales:	Changying Zhao, Zefeng Wei, Junjie Yang, Jiaming Zhang, Chunna Yu, Aijun Yang, Min Zhang, Lin Zhang, Ye Wang, Xiaofeng Mu, Xueyuan Heng, Huijun Yang, Zhongtao Gai, Xuenan Wang, Lei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	vaginal microbiome infertility in vitro fertilization ovulation induction Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/105f2c67cda64e4781fcba8d793a341c
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spelling	oai:doaj.org-article:105f2c67cda64e4781fcba8d793a341c2021-12-02T19:47:35ZCharacterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery10.1128/mSystems.00450-202379-5077https://doaj.org/article/105f2c67cda64e4781fcba8d793a341c2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00450-20https://doaj.org/toc/2379-5077ABSTRACT Perturbation of vaginal microbiome of reproductive-age women influences all the phases of a woman's reproductive life. Although studies have shown that dynamic changes in vaginal microbiome can affect pregnancy, its role in secondary infertility (i.e., inability to become pregnant or to carry a pregnancy successfully after previous success in delivering a child) and in vitro fertilization (IVF) remains to be unraveled. To determine the vaginal microbiome in women undergoing in vitro fertilization and embryo transfer (IVF-ET) and investigate its potential correlations with hormone stimulation, we recruited 30 patients with secondary infertility and receiving IVF and 92 matched healthy women and analyzed their vaginal microbiome composition using 16S rRNA gene sequencing. Our results show that women suffering from infertility (infertile women) exhibit a significant decrease in microbiome diversity and richness compared with healthy women during the nonovulation period (follicular phase) (P < 0.01), whereas vaginal microbiome of healthy women reveals dramatic fluctuations during ovulation (P < 0.05). Interestingly, infertility patients show no change of the vaginal microbiome under conditions of gonadotropin-releasing hormone (GnRH) agonist and recombinant human chorionic gonadotropin (r-hCG) induction (P > 0.05). Moreover, our results indicate that infertile women show characteristic variations in vaginal microbiome, such as increased abundance of Atopobium, Aerococcus, and Bifidobacterium and decreased abundance of Lactobacillus and Leuconostoc. IMPORTANCE The microbiome had been hypothesized to be involved in the physiology and pathophysiology of assisted reproduction before the first success in IVF, while the data supporting or refuting this hypothesis were less than conclusive. Thanks to sequencing data from the 16S rRNA subunit, we characterized the microbiome in the reproductive tract of infertile women, and we found that changes in the vaginal microbiome are related to female infertility. We also found that the characteristic microbiome bacteria are mainly members of several genera and that the vaginal microbiome of infertile women is not sensitive to hormonal changes during IVF. In conclusion, our report provides data that can be used for discovering the role of the vaginal microbiome in patients suffering from secondary infertility.Changying ZhaoZefeng WeiJunjie YangJiaming ZhangChunna YuAijun YangMin ZhangLin ZhangYe WangXiaofeng MuXueyuan HengHuijun YangZhongtao GaiXuenan WangLei ZhangAmerican Society for Microbiologyarticlevaginal microbiomeinfertilityin vitro fertilizationovulation inductionMicrobiologyQR1-502ENmSystems, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaginal microbiome infertility in vitro fertilization ovulation induction Microbiology QR1-502
spellingShingle	vaginal microbiome infertility in vitro fertilization ovulation induction Microbiology QR1-502 Changying Zhao Zefeng Wei Junjie Yang Jiaming Zhang Chunna Yu Aijun Yang Min Zhang Lin Zhang Ye Wang Xiaofeng Mu Xueyuan Heng Huijun Yang Zhongtao Gai Xuenan Wang Lei Zhang Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
description	ABSTRACT Perturbation of vaginal microbiome of reproductive-age women influences all the phases of a woman's reproductive life. Although studies have shown that dynamic changes in vaginal microbiome can affect pregnancy, its role in secondary infertility (i.e., inability to become pregnant or to carry a pregnancy successfully after previous success in delivering a child) and in vitro fertilization (IVF) remains to be unraveled. To determine the vaginal microbiome in women undergoing in vitro fertilization and embryo transfer (IVF-ET) and investigate its potential correlations with hormone stimulation, we recruited 30 patients with secondary infertility and receiving IVF and 92 matched healthy women and analyzed their vaginal microbiome composition using 16S rRNA gene sequencing. Our results show that women suffering from infertility (infertile women) exhibit a significant decrease in microbiome diversity and richness compared with healthy women during the nonovulation period (follicular phase) (P < 0.01), whereas vaginal microbiome of healthy women reveals dramatic fluctuations during ovulation (P < 0.05). Interestingly, infertility patients show no change of the vaginal microbiome under conditions of gonadotropin-releasing hormone (GnRH) agonist and recombinant human chorionic gonadotropin (r-hCG) induction (P > 0.05). Moreover, our results indicate that infertile women show characteristic variations in vaginal microbiome, such as increased abundance of Atopobium, Aerococcus, and Bifidobacterium and decreased abundance of Lactobacillus and Leuconostoc. IMPORTANCE The microbiome had been hypothesized to be involved in the physiology and pathophysiology of assisted reproduction before the first success in IVF, while the data supporting or refuting this hypothesis were less than conclusive. Thanks to sequencing data from the 16S rRNA subunit, we characterized the microbiome in the reproductive tract of infertile women, and we found that changes in the vaginal microbiome are related to female infertility. We also found that the characteristic microbiome bacteria are mainly members of several genera and that the vaginal microbiome of infertile women is not sensitive to hormonal changes during IVF. In conclusion, our report provides data that can be used for discovering the role of the vaginal microbiome in patients suffering from secondary infertility.
format	article
author	Changying Zhao Zefeng Wei Junjie Yang Jiaming Zhang Chunna Yu Aijun Yang Min Zhang Lin Zhang Ye Wang Xiaofeng Mu Xueyuan Heng Huijun Yang Zhongtao Gai Xuenan Wang Lei Zhang
author_facet	Changying Zhao Zefeng Wei Junjie Yang Jiaming Zhang Chunna Yu Aijun Yang Min Zhang Lin Zhang Ye Wang Xiaofeng Mu Xueyuan Heng Huijun Yang Zhongtao Gai Xuenan Wang Lei Zhang
author_sort	Changying Zhao
title	Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
title_short	Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
title_full	Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
title_fullStr	Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
title_full_unstemmed	Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery
title_sort	characterization of the vaginal microbiome in women with infertility and its potential correlation with hormone stimulation during <italic toggle="yes">in vitro</italic> fertilization surgery
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/105f2c67cda64e4781fcba8d793a341c
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Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during <italic toggle="yes">In Vitro</italic> Fertilization Surgery

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