CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells

Acute myeloblastic leukemia (AML) is a disease with a high rate of relapse and drug resistance due to the remaining leukemic stem cells (LSCs). Therefore, LSCs are specific targets for the treatment of leukemia. CD123 is specifically expressed on LSCs and performs as a specific marker. Curcumin is t...

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Autores principales:	Wariya Nirachonkul, Siriporn Ogonoki, Tarika Thumvijit, Supanimit Chiampanichayakul, Pawaret Panyajai, Songyot Anuchapreeda, Singkome Tima, Sawitree Chiampanichayakul
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anti-CD123 leukemic stem cells curcumin acute myeloblastic leukemia nanoparticles Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1060137960654519a4dd0b09051af973
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spelling	oai:doaj.org-article:1060137960654519a4dd0b09051af9732021-11-25T18:31:21ZCD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells10.3390/nano111129742079-4991https://doaj.org/article/1060137960654519a4dd0b09051af9732021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2974https://doaj.org/toc/2079-4991Acute myeloblastic leukemia (AML) is a disease with a high rate of relapse and drug resistance due to the remaining leukemic stem cells (LSCs). Therefore, LSCs are specific targets for the treatment of leukemia. CD123 is specifically expressed on LSCs and performs as a specific marker. Curcumin is the main active compound of a natural product with low toxicity for humans. It has been reported to inhibit leukemic cell growth. However, curcumin is practically insoluble in water and has low bioavailability. In this study, we aimed to formulate curcumin nanoparticles and conjugate with the anti-CD123 to overcome the low water solubility and improve the targeting of LSCs. The cytotoxicity of both curcumin-loaded PLGA/poloxamer nanoparticles (Cur-NPs) and anti-CD123-curcumin-loaded PLGA/poloxamer nanoparticles (anti-CD123-Cur-NPs) were examined in KG-1a cells. The results showed that Cur-NPs and Cur-NPs-CD123 exhibited cytotoxic effects on KG-1a cells with the IC<sub>50</sub> values of 74.20 ± 6.71 and 41.45 ± 5.49 µM, respectively. Moreover, anti-CD123-Cur-NPs induced higher apoptosis than Cur-NPs. The higher uptake of anti-CD123-Cur-NPs in KG-1a cells was confirmed by using flow cytometry. In conclusion, the anti-CD123-Cur-NPs formulation improved curcumin’s bioavailability and specific targeting of LSCs, suggesting that it is a promising drug delivery system for improving the therapeutic efficacy against AML.Wariya NirachonkulSiriporn OgonokiTarika ThumvijitSupanimit ChiampanichayakulPawaret PanyajaiSongyot AnuchapreedaSingkome TimaSawitree ChiampanichayakulMDPI AGarticleanti-CD123leukemic stem cellscurcuminacute myeloblastic leukemiananoparticlesChemistryQD1-999ENNanomaterials, Vol 11, Iss 2974, p 2974 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-CD123 leukemic stem cells curcumin acute myeloblastic leukemia nanoparticles Chemistry QD1-999
spellingShingle	anti-CD123 leukemic stem cells curcumin acute myeloblastic leukemia nanoparticles Chemistry QD1-999 Wariya Nirachonkul Siriporn Ogonoki Tarika Thumvijit Supanimit Chiampanichayakul Pawaret Panyajai Songyot Anuchapreeda Singkome Tima Sawitree Chiampanichayakul CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
description	Acute myeloblastic leukemia (AML) is a disease with a high rate of relapse and drug resistance due to the remaining leukemic stem cells (LSCs). Therefore, LSCs are specific targets for the treatment of leukemia. CD123 is specifically expressed on LSCs and performs as a specific marker. Curcumin is the main active compound of a natural product with low toxicity for humans. It has been reported to inhibit leukemic cell growth. However, curcumin is practically insoluble in water and has low bioavailability. In this study, we aimed to formulate curcumin nanoparticles and conjugate with the anti-CD123 to overcome the low water solubility and improve the targeting of LSCs. The cytotoxicity of both curcumin-loaded PLGA/poloxamer nanoparticles (Cur-NPs) and anti-CD123-curcumin-loaded PLGA/poloxamer nanoparticles (anti-CD123-Cur-NPs) were examined in KG-1a cells. The results showed that Cur-NPs and Cur-NPs-CD123 exhibited cytotoxic effects on KG-1a cells with the IC<sub>50</sub> values of 74.20 ± 6.71 and 41.45 ± 5.49 µM, respectively. Moreover, anti-CD123-Cur-NPs induced higher apoptosis than Cur-NPs. The higher uptake of anti-CD123-Cur-NPs in KG-1a cells was confirmed by using flow cytometry. In conclusion, the anti-CD123-Cur-NPs formulation improved curcumin’s bioavailability and specific targeting of LSCs, suggesting that it is a promising drug delivery system for improving the therapeutic efficacy against AML.
format	article
author	Wariya Nirachonkul Siriporn Ogonoki Tarika Thumvijit Supanimit Chiampanichayakul Pawaret Panyajai Songyot Anuchapreeda Singkome Tima Sawitree Chiampanichayakul
author_facet	Wariya Nirachonkul Siriporn Ogonoki Tarika Thumvijit Supanimit Chiampanichayakul Pawaret Panyajai Songyot Anuchapreeda Singkome Tima Sawitree Chiampanichayakul
author_sort	Wariya Nirachonkul
title	CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
title_short	CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
title_full	CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
title_fullStr	CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
title_full_unstemmed	CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells
title_sort	cd123-targeted nano-curcumin molecule enhances cytotoxic efficacy in leukemic stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1060137960654519a4dd0b09051af973
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_version_	1718410998844489728

CD123-Targeted Nano-Curcumin Molecule Enhances Cytotoxic Efficacy in Leukemic Stem Cells

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