<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism

ABSTRACT Neisseria meningitidis (the meningococcus) is primarily a commensal of the human oropharynx that sporadically causes septicemia and meningitis. Meningococci adapt to diverse local host conditions differing in nutrient supply, like the nasopharynx, blood, and cerebrospinal fluid, by changing...

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Autores principales:	Yvonne Pannekoek, Robert A. G. Huis in ‘t Veld, Kim Schipper, Sandra Bovenkerk, Gertjan Kramer, Matthijs C. Brouwer, Diederik van de Beek, Dave Speijer, Arie van der Ende
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Neisseria meningitidis metabolic regulation pathogenesis sibling sRNAs small regulatory RNAs stringent response Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1068103c365d40b8972224c78c7119cd
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spelling	oai:doaj.org-article:1068103c365d40b8972224c78c7119cd2021-11-15T15:50:59Z<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism10.1128/mBio.02293-162150-7511https://doaj.org/article/1068103c365d40b8972224c78c7119cd2017-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02293-16https://doaj.org/toc/2150-7511ABSTRACT Neisseria meningitidis (the meningococcus) is primarily a commensal of the human oropharynx that sporadically causes septicemia and meningitis. Meningococci adapt to diverse local host conditions differing in nutrient supply, like the nasopharynx, blood, and cerebrospinal fluid, by changing metabolism and protein repertoire. However, regulatory transcription factors and two-component systems in meningococci involved in adaptation to local nutrient variations are limited. We identified novel sibling small regulatory RNAs (Neisseriametabolic switch regulators [NmsRs]) regulating switches between cataplerotic and anaplerotic metabolism in this pathogen. Overexpression of NmsRs was tolerated in blood but not in cerebrospinal fluid. Expression of six tricarboxylic acid cycle enzymes was downregulated by direct action of NmsRs. Expression of the NmsRs themselves was under the control of the stringent response through the action of RelA. Small sibling regulatory RNAs of meningococci, controlling general metabolic switches, add an exciting twist to their versatile repertoire in bacterial pathogens. IMPORTANCE Regulatory small RNAs (sRNAs) of pathogens are coming to be recognized as highly important components of riboregulatory networks, involved in the control of essential cellular processes. They play a prominent role in adaptation to physiological changes as represented by different host environments. They can function as posttranscriptional regulators of gene expression to orchestrate metabolic adaptation to nutrient stresses. Here, we identified highly conserved sibling sRNAs in Neisseria meningitidis which are functionally involved in the regulation of gene expression of components of the tricarboxylic acid cycle. These novel sibling sRNAs that function by antisense mechanisms extend the so-called stringent response which connects metabolic status to colonization and possibly virulence as well as pathogenesis in meningococci.Yvonne PannekoekRobert A. G. Huis in ‘t VeldKim SchipperSandra BovenkerkGertjan KramerMatthijs C. BrouwerDiederik van de BeekDave SpeijerArie van der EndeAmerican Society for MicrobiologyarticleNeisseria meningitidismetabolic regulationpathogenesissibling sRNAssmall regulatory RNAsstringent responseMicrobiologyQR1-502ENmBio, Vol 8, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neisseria meningitidis metabolic regulation pathogenesis sibling sRNAs small regulatory RNAs stringent response Microbiology QR1-502
spellingShingle	Neisseria meningitidis metabolic regulation pathogenesis sibling sRNAs small regulatory RNAs stringent response Microbiology QR1-502 Yvonne Pannekoek Robert A. G. Huis in ‘t Veld Kim Schipper Sandra Bovenkerk Gertjan Kramer Matthijs C. Brouwer Diederik van de Beek Dave Speijer Arie van der Ende <italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
description	ABSTRACT Neisseria meningitidis (the meningococcus) is primarily a commensal of the human oropharynx that sporadically causes septicemia and meningitis. Meningococci adapt to diverse local host conditions differing in nutrient supply, like the nasopharynx, blood, and cerebrospinal fluid, by changing metabolism and protein repertoire. However, regulatory transcription factors and two-component systems in meningococci involved in adaptation to local nutrient variations are limited. We identified novel sibling small regulatory RNAs (Neisseriametabolic switch regulators [NmsRs]) regulating switches between cataplerotic and anaplerotic metabolism in this pathogen. Overexpression of NmsRs was tolerated in blood but not in cerebrospinal fluid. Expression of six tricarboxylic acid cycle enzymes was downregulated by direct action of NmsRs. Expression of the NmsRs themselves was under the control of the stringent response through the action of RelA. Small sibling regulatory RNAs of meningococci, controlling general metabolic switches, add an exciting twist to their versatile repertoire in bacterial pathogens. IMPORTANCE Regulatory small RNAs (sRNAs) of pathogens are coming to be recognized as highly important components of riboregulatory networks, involved in the control of essential cellular processes. They play a prominent role in adaptation to physiological changes as represented by different host environments. They can function as posttranscriptional regulators of gene expression to orchestrate metabolic adaptation to nutrient stresses. Here, we identified highly conserved sibling sRNAs in Neisseria meningitidis which are functionally involved in the regulation of gene expression of components of the tricarboxylic acid cycle. These novel sibling sRNAs that function by antisense mechanisms extend the so-called stringent response which connects metabolic status to colonization and possibly virulence as well as pathogenesis in meningococci.
format	article
author	Yvonne Pannekoek Robert A. G. Huis in ‘t Veld Kim Schipper Sandra Bovenkerk Gertjan Kramer Matthijs C. Brouwer Diederik van de Beek Dave Speijer Arie van der Ende
author_facet	Yvonne Pannekoek Robert A. G. Huis in ‘t Veld Kim Schipper Sandra Bovenkerk Gertjan Kramer Matthijs C. Brouwer Diederik van de Beek Dave Speijer Arie van der Ende
author_sort	Yvonne Pannekoek
title	<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
title_short	<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
title_full	<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
title_fullStr	<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
title_full_unstemmed	<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism
title_sort	<italic toggle="yes">neisseria meningitidis</italic> uses sibling small regulatory rnas to switch from cataplerotic to anaplerotic metabolism
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/1068103c365d40b8972224c78c7119cd
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<italic toggle="yes">Neisseria meningitidis</italic> Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism

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