Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.

Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in v...

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Autores principales:	Nuno Palha, Florence Guivel-Benhassine, Valérie Briolat, Georges Lutfalla, Marion Sourisseau, Felix Ellett, Chieh-Huei Wang, Graham J Lieschke, Philippe Herbomel, Olivier Schwartz, Jean-Pierre Levraud
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1068793baf1541c788ca459b9ddce8a9
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id	oai:doaj.org-article:1068793baf1541c788ca459b9ddce8a9
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spelling	oai:doaj.org-article:1068793baf1541c788ca459b9ddce8a92021-11-18T06:07:40ZReal-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.1553-73661553-737410.1371/journal.ppat.1003619https://doaj.org/article/1068793baf1541c788ca459b9ddce8a92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039582/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.Nuno PalhaFlorence Guivel-BenhassineValérie BriolatGeorges LutfallaMarion SourisseauFelix EllettChieh-Huei WangGraham J LieschkePhilippe HerbomelOlivier SchwartzJean-Pierre LevraudPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 9, p e1003619 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Nuno Palha Florence Guivel-Benhassine Valérie Briolat Georges Lutfalla Marion Sourisseau Felix Ellett Chieh-Huei Wang Graham J Lieschke Philippe Herbomel Olivier Schwartz Jean-Pierre Levraud Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
description	Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
format	article
author	Nuno Palha Florence Guivel-Benhassine Valérie Briolat Georges Lutfalla Marion Sourisseau Felix Ellett Chieh-Huei Wang Graham J Lieschke Philippe Herbomel Olivier Schwartz Jean-Pierre Levraud
author_facet	Nuno Palha Florence Guivel-Benhassine Valérie Briolat Georges Lutfalla Marion Sourisseau Felix Ellett Chieh-Huei Wang Graham J Lieschke Philippe Herbomel Olivier Schwartz Jean-Pierre Levraud
author_sort	Nuno Palha
title	Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
title_short	Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
title_full	Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
title_fullStr	Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
title_full_unstemmed	Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
title_sort	real-time whole-body visualization of chikungunya virus infection and host interferon response in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1068793baf1541c788ca459b9ddce8a9
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Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.

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