Characterization of family IV UDG from Aeropyrum pernix and its application in hot-start PCR by family B DNA polymerase.
Recombinant uracil-DNA glycosylase (UDG) from Aeropyrum pernix (A. pernix) was expressed in E. coli. The biochemical characteristics of A. pernix UDG (ApeUDG) were studied using oligonucleotides carrying a deoxyuracil (dU) base. The optimal temperature range and pH value for dU removal by ApeUDG wer...
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| Auteurs principaux: | , |
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| Format: | article |
| Langue: | EN |
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Public Library of Science (PLoS)
2011
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| Accès en ligne: | https://doaj.org/article/106c9a11192d41ff980582234ebfd92b |
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