PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION

Since 1924, BCG vaccine is used to protect children from the most severe forms of tuberculosis. At the same time, the protective effect of BCG in adults is variable. The potential for revaccination with live vaccine is further limited by the rapid spread of HIV infection. The early-secreted Mycobact...

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Autores principales:	V. V. Yeremeev, G. S. Shepelkova, I. V. Dukhovlinov, V. Ya. Gergert
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	tuberculosis vaccine bcg boost immunization Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/106f0cdfdf5d42be9b453ae931a7cb92
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spelling	oai:doaj.org-article:106f0cdfdf5d42be9b453ae931a7cb922021-11-18T08:03:48ZPROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION1563-06252313-741X10.15789/1563-0625-2019-3-555-558https://doaj.org/article/106f0cdfdf5d42be9b453ae931a7cb922019-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1829https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XSince 1924, BCG vaccine is used to protect children from the most severe forms of tuberculosis. At the same time, the protective effect of BCG in adults is variable. The potential for revaccination with live vaccine is further limited by the rapid spread of HIV infection. The early-secreted Mycobacterium tuberculosis proteins have been used extensively in TB vaccine development, due to their high immunogenicity and have shown protective effect in animal models. The aim of our study was to evaluate the opportunity to increase the anti-TB resistance in experimental animals by re-vaccination with a new subunit vaccine preparation following primary immunization with BCG. To perform such boost vaccination, we used a combination of the Ag85B-TB10.4-FliC chimeric protein, and the plasmid DNA encoding Ag85A antigen. Efficiency of the boost vaccination was evaluated in a model of M. tuberculosis H37Rv aerosol infection of C57BL / 6 laboratory mice, either in the intact animals, or those vaccinated with BCG only, or BCG followed by revaccination with the test vaccine. The data concerning mycobacteria outgrowth from the organs, and life-span of animals after infection were subject to comparative analysis. We have demonstrated that additional boost vaccination with the vaccine under study, as compared with conventional BCG vaccination, leads to further inhibition of mycobacteria dissemination from the site of infection, and significantly prolonged survival of infected animals.V. V. YeremeevG. S. ShepelkovaI. V. DukhovlinovV. Ya. GergertSPb RAACIarticletuberculosisvaccinebcgboost immunizationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 3, Pp 555-558 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	tuberculosis vaccine bcg boost immunization Immunologic diseases. Allergy RC581-607
spellingShingle	tuberculosis vaccine bcg boost immunization Immunologic diseases. Allergy RC581-607 V. V. Yeremeev G. S. Shepelkova I. V. Dukhovlinov V. Ya. Gergert PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
description	Since 1924, BCG vaccine is used to protect children from the most severe forms of tuberculosis. At the same time, the protective effect of BCG in adults is variable. The potential for revaccination with live vaccine is further limited by the rapid spread of HIV infection. The early-secreted Mycobacterium tuberculosis proteins have been used extensively in TB vaccine development, due to their high immunogenicity and have shown protective effect in animal models. The aim of our study was to evaluate the opportunity to increase the anti-TB resistance in experimental animals by re-vaccination with a new subunit vaccine preparation following primary immunization with BCG. To perform such boost vaccination, we used a combination of the Ag85B-TB10.4-FliC chimeric protein, and the plasmid DNA encoding Ag85A antigen. Efficiency of the boost vaccination was evaluated in a model of M. tuberculosis H37Rv aerosol infection of C57BL / 6 laboratory mice, either in the intact animals, or those vaccinated with BCG only, or BCG followed by revaccination with the test vaccine. The data concerning mycobacteria outgrowth from the organs, and life-span of animals after infection were subject to comparative analysis. We have demonstrated that additional boost vaccination with the vaccine under study, as compared with conventional BCG vaccination, leads to further inhibition of mycobacteria dissemination from the site of infection, and significantly prolonged survival of infected animals.
format	article
author	V. V. Yeremeev G. S. Shepelkova I. V. Dukhovlinov V. Ya. Gergert
author_facet	V. V. Yeremeev G. S. Shepelkova I. V. Dukhovlinov V. Ya. Gergert
author_sort	V. V. Yeremeev
title	PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
title_short	PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
title_full	PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
title_fullStr	PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
title_full_unstemmed	PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION
title_sort	protective effect induced by the new subunit tuberculosis vaccine when used as a bcg boost is associated with inhibition of mycobacterial dissemination
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/106f0cdfdf5d42be9b453ae931a7cb92
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PROTECTIVE EFFECT INDUCED BY THE NEW SUBUNIT TUBERCULOSIS VACCINE WHEN USED AS A BCG BOOST IS ASSOCIATED WITH INHIBITION OF MYCOBACTERIAL DISSEMINATION

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