Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)

Taiwo Olayemi Elufioye,1 Efere M Obuotor,2 Joseph M Agbedahunsi,3 Saburi A Adesanya4 1Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, 2Department of Biochemistry, 3Drug Research and Production Unit, Faculty of Pharmacy, 4Department of Pharmacognosy, Faculty of Pharmac...

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Autores principales:	Elufioye TO, Obuotor EM, Agbedahunsi JM, Adesanya SA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Alzheimer’s disease (AD) Acetylcholinesterase butyrylcholinesterase neurodegenerative Spondias mombin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/10704b775ba7463f830a9401ab9fe96f
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spelling	oai:doaj.org-article:10704b775ba7463f830a9401ab9fe96f2021-12-02T02:17:02ZAnticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)1177-5491https://doaj.org/article/10704b775ba7463f830a9401ab9fe96f2017-08-01T00:00:00Zhttps://www.dovepress.com/anticholinesterase-constituents-from-the-leaves-of-spondias-mombin-l-a-peer-reviewed-article-BTThttps://doaj.org/toc/1177-5491Taiwo Olayemi Elufioye,1 Efere M Obuotor,2 Joseph M Agbedahunsi,3 Saburi A Adesanya4 1Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, 2Department of Biochemistry, 3Drug Research and Production Unit, Faculty of Pharmacy, 4Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Osun, Nigeria Abstract: Spondias mombin has been used in traditional medicine for the management of several diseases, including memory loss. This study aimed to evaluate the cholinesterase inhibitory activity of the methanol extract of the leaves and its derived fractions, as well as carry out detailed phytochemical investigations leading to the isolation and characterization of bioactive compounds from the plant. The acetyl cholinesterase (AChE) and butyryl cholinesterase (BUChE) inhibitory activities were evaluated by colorimetric and thin-layer chromatography bioautographic assay techniques. The ethyl acetate fraction was most active against both enzymes, with percentage inhibition of 58.10 ± 1.08% and 52.66 ± 1.34% against AChE and BUChE, respectively. Three compounds, namely, botulin, campesterol and phytol, with IC50 of 0.88 μg/mL (AChE), 4.67 μg/mL (BuChE); 1.89 μg/mL (AChE), 4.08 μg/mL (BuChE) and 12.51 μg/mL (AChE), 23.89 μg/mL (BuChE), respectively, were isolated from the supernatant of the ethyl acetate fraction. The isolated cholinesterase inhibitory compounds correlate with the known memory-enhancing property of the plant and thus support one of its uses in ethnomedicine. Keywords: Alzheimer’s disease, acetylcholinesterase, butyryl cholinesterase, neurodegenerative, Spondias mombin Elufioye TOObuotor EMAgbedahunsi JMAdesanya SADove Medical PressarticleAlzheimer’s disease (AD)AcetylcholinesterasebutyrylcholinesteraseneurodegenerativeSpondias mombinMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol Volume 11, Pp 107-114 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer’s disease (AD) Acetylcholinesterase butyrylcholinesterase neurodegenerative Spondias mombin Medicine (General) R5-920
spellingShingle	Alzheimer’s disease (AD) Acetylcholinesterase butyrylcholinesterase neurodegenerative Spondias mombin Medicine (General) R5-920 Elufioye TO Obuotor EM Agbedahunsi JM Adesanya SA Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
description	Taiwo Olayemi Elufioye,1 Efere M Obuotor,2 Joseph M Agbedahunsi,3 Saburi A Adesanya4 1Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, 2Department of Biochemistry, 3Drug Research and Production Unit, Faculty of Pharmacy, 4Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Osun, Nigeria Abstract: Spondias mombin has been used in traditional medicine for the management of several diseases, including memory loss. This study aimed to evaluate the cholinesterase inhibitory activity of the methanol extract of the leaves and its derived fractions, as well as carry out detailed phytochemical investigations leading to the isolation and characterization of bioactive compounds from the plant. The acetyl cholinesterase (AChE) and butyryl cholinesterase (BUChE) inhibitory activities were evaluated by colorimetric and thin-layer chromatography bioautographic assay techniques. The ethyl acetate fraction was most active against both enzymes, with percentage inhibition of 58.10 ± 1.08% and 52.66 ± 1.34% against AChE and BUChE, respectively. Three compounds, namely, botulin, campesterol and phytol, with IC50 of 0.88 μg/mL (AChE), 4.67 μg/mL (BuChE); 1.89 μg/mL (AChE), 4.08 μg/mL (BuChE) and 12.51 μg/mL (AChE), 23.89 μg/mL (BuChE), respectively, were isolated from the supernatant of the ethyl acetate fraction. The isolated cholinesterase inhibitory compounds correlate with the known memory-enhancing property of the plant and thus support one of its uses in ethnomedicine. Keywords: Alzheimer’s disease, acetylcholinesterase, butyryl cholinesterase, neurodegenerative, Spondias mombin
format	article
author	Elufioye TO Obuotor EM Agbedahunsi JM Adesanya SA
author_facet	Elufioye TO Obuotor EM Agbedahunsi JM Adesanya SA
author_sort	Elufioye TO
title	Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
title_short	Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
title_full	Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
title_fullStr	Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
title_full_unstemmed	Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)
title_sort	anticholinesterase constituents from the leaves of spondias mombin l. (anacardiaceae)
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/10704b775ba7463f830a9401ab9fe96f
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Anticholinesterase constituents from the leaves of Spondias mombin L. (Anacardiaceae)

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