The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

Yunzhu Qian,1,2 Hanbang Chen,1 Yang Xu,1 Jianxin Yang,2 Xuefeng Zhou,3 Feimin Zhang,1 Ning Gu3 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 2Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou, 3School of Biological Science and Medic...

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Autores principales:	Qian YZ, Chen HB, Xu Y, Yang JX, Zhou XF, Zhang FM, Gu N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Guided bone regeneration Biomimetic architecture Bioactive molecular Preosteoblast response Collagen. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1072f26b50354f039b16ebe06d17b036
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spelling	oai:doaj.org-article:1072f26b50354f039b16ebe06d17b0362021-12-02T05:36:18ZThe preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I1178-2013https://doaj.org/article/1072f26b50354f039b16ebe06d17b0362016-08-01T00:00:00Zhttps://www.dovepress.com/the-preosteoblast-response-of-electrospinning-plgapcl-nanofibers-effec-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yunzhu Qian,1,2 Hanbang Chen,1 Yang Xu,1 Jianxin Yang,2 Xuefeng Zhou,3 Feimin Zhang,1 Ning Gu3 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 2Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou, 3School of Biological Science and Medical Engineering, Southeast University, Nanjing, People’s Republic of China Abstract: Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation. Keywords: guided bone regeneration, biomimetic architecture, bioactive molecular, preosteoblast response, collagenQian YZChen HBXu YYang JXZhou XFZhang FMGu NDove Medical PressarticleGuided bone regenerationBiomimetic architectureBioactive molecularPreosteoblast responseCollagen.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4157-4171 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Guided bone regeneration Biomimetic architecture Bioactive molecular Preosteoblast response Collagen. Medicine (General) R5-920
spellingShingle	Guided bone regeneration Biomimetic architecture Bioactive molecular Preosteoblast response Collagen. Medicine (General) R5-920 Qian YZ Chen HB Xu Y Yang JX Zhou XF Zhang FM Gu N The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
description	Yunzhu Qian,1,2 Hanbang Chen,1 Yang Xu,1 Jianxin Yang,2 Xuefeng Zhou,3 Feimin Zhang,1 Ning Gu3 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 2Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou, 3School of Biological Science and Medical Engineering, Southeast University, Nanjing, People’s Republic of China Abstract: Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation. Keywords: guided bone regeneration, biomimetic architecture, bioactive molecular, preosteoblast response, collagen
format	article
author	Qian YZ Chen HB Xu Y Yang JX Zhou XF Zhang FM Gu N
author_facet	Qian YZ Chen HB Xu Y Yang JX Zhou XF Zhang FM Gu N
author_sort	Qian YZ
title	The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
title_short	The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
title_full	The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
title_fullStr	The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
title_full_unstemmed	The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I
title_sort	preosteoblast response of electrospinning plga/pcl nanofibers: effects of biomimetic architecture and collagen i
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/1072f26b50354f039b16ebe06d17b036
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The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

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