Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia

Abstract. Madduppa H, Putri ASP, Wicaksono RZ, Subhan B, Akbar N, Ismail F, Arafat D, Prabuning D, Sani LMI, Srimariana ES, Baksir A, Bengen DG. 2020. Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia. Biodiversitas 21...

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Autores principales:	Hawis MADDUPPA, ARINI S. P. PUTRI, RIDHO Z. WICAKSONO, BEGINER SUBHAN, NEBUCHADNEZZAR AKBAR, FIRDAUT ISMAIL, DONDY ARAFAT, DERTA PRABUNING, L.M. IQBAL MUKSIN, ENDANG S. SRIMARIANA, ABDURRACHMAN BAKSIR, DIETRIECH G. BENGEN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	complex species, coral triangle, endemic species, length-weight relationship, phylogenetic reconstruction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1075a82e7ade47f0a5beaa61bb45ea36
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spelling	oai:doaj.org-article:1075a82e7ade47f0a5beaa61bb45ea362021-11-22T00:40:51ZMorphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia1412-033X2085-472210.13057/biodiv/d210757https://doaj.org/article/1075a82e7ade47f0a5beaa61bb45ea362020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5869https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Madduppa H, Putri ASP, Wicaksono RZ, Subhan B, Akbar N, Ismail F, Arafat D, Prabuning D, Sani LMI, Srimariana ES, Baksir A, Bengen DG. 2020. Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia. Biodiversitas 21: 3331-3343. The walking shark (Hemiscyllium halmahera) commonly referred to as bamboo shark, is a shark species that belongs to the Hemiscyllidae family which is endemic in North Maluku, and Indonesian waters. These Hemiscyllium species are generally confined to the Australian-New Guinea region, save a specie which originates from Halmahera, an Indonesian island. Ever since its discovery, sufficient information on its existence has been largely unavailable. Hence, the purpose of this study is (1) to assess the morphology, using meristic measurements and length-weight relationship, and (2) to determine the molecular characteristics through DNA barcoding, phylogenetic reconstruction, and genetic diversity. The samples used in this research were obtained from Halmahera Archipelago, North Maluku, including Halmahera, Tidore, Ternate, Maitara, and Guraici in several expeditions between March 2017 and January 2018. 36 individuals in total were measured for morphometric parameters including Total Length (TL), Standard Length (SL), Head Length (HL), Body Circumference (BC), and Body Weight (BW). Out of a total of 36 sharks, tissue samples were obtained from 32 individuals for molecular identification. After the required measurements were obtained, all individuals sharks were released back to their habitat safely. Equations of linear regression were used to analyze the relationship between length and weight. Morphometric measurements indicated that the average total length of the walking shark samples obtained, were not more than 75± cm for both male and female specimens. The length-weight analysis revealed a positive allometric growth pattern in H. halmahera species, where weight was gained faster than an increase in length. The results of the DNA fragment length analysis from all samples were 793-bp. All 32 tissue samples identified as H. halmahera consisted of 3 samples from Ternate Island, 14 samples from Maitara Island, 9 samples from Guraici Island (South Halmahera), 10 samples from Central Halmahera, and 2 samples from Tidore Island. These results confirmed that DNA subsequence analysis indicates that H. halmahera is different from those sequences extracted from Genbank in the Australia-New Guinea region. This study successfully identified H. halmahera based on their morphological and molecular characters, and has therefore contributed to international genetic database (Barcoding Of Life Database-BOLD) for further studies.Hawis MADDUPPAARINI S. P. PUTRIRIDHO Z. WICAKSONOBEGINER SUBHANNEBUCHADNEZZAR AKBARFIRDAUT ISMAILDONDY ARAFATDERTA PRABUNINGL.M. IQBAL MUKSINENDANG S. SRIMARIANAABDURRACHMAN BAKSIRDIETRIECH G. BENGENMBI & UNS Soloarticlecomplex species, coral triangle, endemic species, length-weight relationship, phylogenetic reconstructionBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	complex species, coral triangle, endemic species, length-weight relationship, phylogenetic reconstruction Biology (General) QH301-705.5
spellingShingle	complex species, coral triangle, endemic species, length-weight relationship, phylogenetic reconstruction Biology (General) QH301-705.5 Hawis MADDUPPA ARINI S. P. PUTRI RIDHO Z. WICAKSONO BEGINER SUBHAN NEBUCHADNEZZAR AKBAR FIRDAUT ISMAIL DONDY ARAFAT DERTA PRABUNING L.M. IQBAL MUKSIN ENDANG S. SRIMARIANA ABDURRACHMAN BAKSIR DIETRIECH G. BENGEN Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
description	Abstract. Madduppa H, Putri ASP, Wicaksono RZ, Subhan B, Akbar N, Ismail F, Arafat D, Prabuning D, Sani LMI, Srimariana ES, Baksir A, Bengen DG. 2020. Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia. Biodiversitas 21: 3331-3343. The walking shark (Hemiscyllium halmahera) commonly referred to as bamboo shark, is a shark species that belongs to the Hemiscyllidae family which is endemic in North Maluku, and Indonesian waters. These Hemiscyllium species are generally confined to the Australian-New Guinea region, save a specie which originates from Halmahera, an Indonesian island. Ever since its discovery, sufficient information on its existence has been largely unavailable. Hence, the purpose of this study is (1) to assess the morphology, using meristic measurements and length-weight relationship, and (2) to determine the molecular characteristics through DNA barcoding, phylogenetic reconstruction, and genetic diversity. The samples used in this research were obtained from Halmahera Archipelago, North Maluku, including Halmahera, Tidore, Ternate, Maitara, and Guraici in several expeditions between March 2017 and January 2018. 36 individuals in total were measured for morphometric parameters including Total Length (TL), Standard Length (SL), Head Length (HL), Body Circumference (BC), and Body Weight (BW). Out of a total of 36 sharks, tissue samples were obtained from 32 individuals for molecular identification. After the required measurements were obtained, all individuals sharks were released back to their habitat safely. Equations of linear regression were used to analyze the relationship between length and weight. Morphometric measurements indicated that the average total length of the walking shark samples obtained, were not more than 75± cm for both male and female specimens. The length-weight analysis revealed a positive allometric growth pattern in H. halmahera species, where weight was gained faster than an increase in length. The results of the DNA fragment length analysis from all samples were 793-bp. All 32 tissue samples identified as H. halmahera consisted of 3 samples from Ternate Island, 14 samples from Maitara Island, 9 samples from Guraici Island (South Halmahera), 10 samples from Central Halmahera, and 2 samples from Tidore Island. These results confirmed that DNA subsequence analysis indicates that H. halmahera is different from those sequences extracted from Genbank in the Australia-New Guinea region. This study successfully identified H. halmahera based on their morphological and molecular characters, and has therefore contributed to international genetic database (Barcoding Of Life Database-BOLD) for further studies.
format	article
author	Hawis MADDUPPA ARINI S. P. PUTRI RIDHO Z. WICAKSONO BEGINER SUBHAN NEBUCHADNEZZAR AKBAR FIRDAUT ISMAIL DONDY ARAFAT DERTA PRABUNING L.M. IQBAL MUKSIN ENDANG S. SRIMARIANA ABDURRACHMAN BAKSIR DIETRIECH G. BENGEN
author_facet	Hawis MADDUPPA ARINI S. P. PUTRI RIDHO Z. WICAKSONO BEGINER SUBHAN NEBUCHADNEZZAR AKBAR FIRDAUT ISMAIL DONDY ARAFAT DERTA PRABUNING L.M. IQBAL MUKSIN ENDANG S. SRIMARIANA ABDURRACHMAN BAKSIR DIETRIECH G. BENGEN
author_sort	Hawis MADDUPPA
title	Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
title_short	Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
title_full	Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
title_fullStr	Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
title_full_unstemmed	Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
title_sort	morphometric and dna barcoding of endemic halmaheran walking shark (hemiscyllium halmahera, allen, 2013) in north maluku, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/1075a82e7ade47f0a5beaa61bb45ea36
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Morphometric and DNA Barcoding of endemic Halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia

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