Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species

Abstract Chromosomal inversions are structural changes that alter gene order but generally not gene content in the affected region. In Drosophila, extensive cytological studies revealed the widespread character of inversion polymorphism, with evidence for its adaptive character. In Drosophila subobs...

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Autores principales:	Eva Puerma, Dorcas J. Orengo, Montserrat Aguadé
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/107b47aa98db43e1be59d006b9600f82
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spelling	oai:doaj.org-article:107b47aa98db43e1be59d006b9600f822021-12-02T15:05:01ZInversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species10.1038/s41598-017-17650-12045-2322https://doaj.org/article/107b47aa98db43e1be59d006b9600f822017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17650-1https://doaj.org/toc/2045-2322Abstract Chromosomal inversions are structural changes that alter gene order but generally not gene content in the affected region. In Drosophila, extensive cytological studies revealed the widespread character of inversion polymorphism, with evidence for its adaptive character. In Drosophila subobscura, polymorphism affects both its four large autosomal elements and its X (A) chromosome. The characterization of eight of these autosomal inversions breakpoints revealed that most of them originated through the staggered-breaks mechanism. Here, we have performed chromosomal walks to identify the breakpoints of two X-chromosome widely distributed inversions —A2 and A1— of D. subobscura. Inversion A2 is considered a warm-adapted arrangement that exhibits parallel latitudinal clines in the species ancestral distribution area and in both American subcontinents, whereas inversion A1 is only present in the Palearctic region where it presents an east-west cline. The duplication detected at the A2 inversion breakpoints is consistent with its origin by the staggered-breaks mechanism. Inversion A1 breakpoints could not be molecularly identified even though they could be narrowly delimited. This result points to chromosome walking limitations when using as a guide the genome of other species. Limitations stem from the rate of evolution by paracentric inversions, which in Drosophila is highest for the X chromosome.Eva PuermaDorcas J. OrengoMontserrat AguadéNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eva Puerma Dorcas J. Orengo Montserrat Aguadé Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
description	Abstract Chromosomal inversions are structural changes that alter gene order but generally not gene content in the affected region. In Drosophila, extensive cytological studies revealed the widespread character of inversion polymorphism, with evidence for its adaptive character. In Drosophila subobscura, polymorphism affects both its four large autosomal elements and its X (A) chromosome. The characterization of eight of these autosomal inversions breakpoints revealed that most of them originated through the staggered-breaks mechanism. Here, we have performed chromosomal walks to identify the breakpoints of two X-chromosome widely distributed inversions —A2 and A1— of D. subobscura. Inversion A2 is considered a warm-adapted arrangement that exhibits parallel latitudinal clines in the species ancestral distribution area and in both American subcontinents, whereas inversion A1 is only present in the Palearctic region where it presents an east-west cline. The duplication detected at the A2 inversion breakpoints is consistent with its origin by the staggered-breaks mechanism. Inversion A1 breakpoints could not be molecularly identified even though they could be narrowly delimited. This result points to chromosome walking limitations when using as a guide the genome of other species. Limitations stem from the rate of evolution by paracentric inversions, which in Drosophila is highest for the X chromosome.
format	article
author	Eva Puerma Dorcas J. Orengo Montserrat Aguadé
author_facet	Eva Puerma Dorcas J. Orengo Montserrat Aguadé
author_sort	Eva Puerma
title	Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
title_short	Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
title_full	Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
title_fullStr	Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
title_full_unstemmed	Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
title_sort	inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/107b47aa98db43e1be59d006b9600f82
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Inversion evolutionary rates might limit the experimental identification of inversion breakpoints in non-model species

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