Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics

Flavonoid is phenolic compounds consisting of fifteen carbon atoms and is commonly found in plants. Infrared (IR) spectroscopy combined with chemometrics, has been developed for a simple analysis of flavonoid in the medicinal plant leaves powder. IR spectra of selected medicinal plant powder were co...

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Autores principales:	Lestyo Wulandari, Bayu Dwi Permana, Nia Kristiningrum
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2020
Materias:	medicinal plant leaves powder total flavonoid content nir ftir chemometric Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/107c774418a44a62b1355c40f7f6a764
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spelling	oai:doaj.org-article:107c774418a44a62b1355c40f7f6a7642021-12-02T15:37:45ZDetermination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics1411-94202460-157810.22146/ijc.47047https://doaj.org/article/107c774418a44a62b1355c40f7f6a7642020-07-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/47047https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Flavonoid is phenolic compounds consisting of fifteen carbon atoms and is commonly found in plants. Infrared (IR) spectroscopy combined with chemometrics, has been developed for a simple analysis of flavonoid in the medicinal plant leaves powder. IR spectra of selected medicinal plant powder were correlated with flavonoid content using chemometrics. The chemometric methods used for calibration analysis were Partial Least Square (PLS), Principal Component Regression (PCR), and Support Vector Regression (SVR). After the calibration model was formed, it was then validated using Leave-One-Out-Cross-Validation (LOOCV) and 2-fold cross-validation. In this study, the PLS of the Near-infrared (NIR) calibration model showed the best calibration with R-Square and RMSEC values of 0.9676524 and 0.0978202, respectively. The LOOCV of PLS of the NIR calibration model has the R-square and RMSE values of 0.9850164 and 0.067663, respectively. The 2-fold cross-validation gave the R-square and RMSE values of 0.9857071 and 0.2104665, respectively. PLS of the NIR calibration model was further used to predict unknown flavonoid content in commercial samples. The significance of flavonoid content that has been measured by NIR and UV-Vis spectrophotometry was evaluated with paired samples T-test. The flavonoid content that has been measured with both methods gave no significant difference.Lestyo WulandariBayu Dwi PermanaNia KristiningrumDepartment of Chemistry, Universitas Gadjah Madaarticlemedicinal plant leaves powdertotal flavonoid contentnirftirchemometricChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 20, Iss 5, Pp 1044-1051 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	medicinal plant leaves powder total flavonoid content nir ftir chemometric Chemistry QD1-999
spellingShingle	medicinal plant leaves powder total flavonoid content nir ftir chemometric Chemistry QD1-999 Lestyo Wulandari Bayu Dwi Permana Nia Kristiningrum Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
description	Flavonoid is phenolic compounds consisting of fifteen carbon atoms and is commonly found in plants. Infrared (IR) spectroscopy combined with chemometrics, has been developed for a simple analysis of flavonoid in the medicinal plant leaves powder. IR spectra of selected medicinal plant powder were correlated with flavonoid content using chemometrics. The chemometric methods used for calibration analysis were Partial Least Square (PLS), Principal Component Regression (PCR), and Support Vector Regression (SVR). After the calibration model was formed, it was then validated using Leave-One-Out-Cross-Validation (LOOCV) and 2-fold cross-validation. In this study, the PLS of the Near-infrared (NIR) calibration model showed the best calibration with R-Square and RMSEC values of 0.9676524 and 0.0978202, respectively. The LOOCV of PLS of the NIR calibration model has the R-square and RMSE values of 0.9850164 and 0.067663, respectively. The 2-fold cross-validation gave the R-square and RMSE values of 0.9857071 and 0.2104665, respectively. PLS of the NIR calibration model was further used to predict unknown flavonoid content in commercial samples. The significance of flavonoid content that has been measured by NIR and UV-Vis spectrophotometry was evaluated with paired samples T-test. The flavonoid content that has been measured with both methods gave no significant difference.
format	article
author	Lestyo Wulandari Bayu Dwi Permana Nia Kristiningrum
author_facet	Lestyo Wulandari Bayu Dwi Permana Nia Kristiningrum
author_sort	Lestyo Wulandari
title	Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
title_short	Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
title_full	Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
title_fullStr	Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
title_full_unstemmed	Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics
title_sort	determination of total flavonoid content in medicinal plant leaves powder using infrared spectroscopy and chemometrics
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/107c774418a44a62b1355c40f7f6a764
work_keys_str_mv	AT lestyowulandari determinationoftotalflavonoidcontentinmedicinalplantleavespowderusinginfraredspectroscopyandchemometrics AT bayudwipermana determinationoftotalflavonoidcontentinmedicinalplantleavespowderusinginfraredspectroscopyandchemometrics AT niakristiningrum determinationoftotalflavonoidcontentinmedicinalplantleavespowderusinginfraredspectroscopyandchemometrics
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Determination of Total Flavonoid Content in Medicinal Plant Leaves Powder Using Infrared Spectroscopy and Chemometrics

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