A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.

A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer...

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Autores principales:	Hideyuki Terazono, Hyonchol Kim, Masahito Hayashi, Akihiro Hattori, Fumimasa Nomura, Tomoyuki Kaneko, Kenji Yasuda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1083ce7beaf045249e18e7e9a0328fca
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spelling	oai:doaj.org-article:1083ce7beaf045249e18e7e9a0328fca2021-11-18T07:09:49ZA non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.1932-620310.1371/journal.pone.0042485https://doaj.org/article/1083ce7beaf045249e18e7e9a0328fca2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870332/?tool=EBIhttps://doaj.org/toc/1932-6203A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.Hideyuki TerazonoHyonchol KimMasahito HayashiAkihiro HattoriFumimasa NomuraTomoyuki KanekoKenji YasudaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42485 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
description	A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.
format	article
author	Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda
author_facet	Hideyuki Terazono Hyonchol Kim Masahito Hayashi Akihiro Hattori Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda
author_sort	Hideyuki Terazono
title	A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
title_short	A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
title_full	A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
title_fullStr	A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
title_full_unstemmed	A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
title_sort	non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1083ce7beaf045249e18e7e9a0328fca
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A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.

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