A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer.
A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer...
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| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Public Library of Science (PLoS)
2012
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| Subjects: | |
| Online Access: | https://doaj.org/article/1083ce7beaf045249e18e7e9a0328fca |
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