Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play importan...

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Autores principales:	Hasan Nasrullah, Yanti Inneke Nababan, Ika Safitri, Dwi Yanti, Sri Nuryati, Muhammad Zairin Jr, Alimuddin Alimuddin
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	aeromonas hydrophila, african catfish, disease resistance, lysozyme gene, snp Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/10847ec77bdb4cb5bac72189c83bfeeb
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spelling	oai:doaj.org-article:10847ec77bdb4cb5bac72189c83bfeeb2021-11-21T22:09:24ZShort Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus1412-033X2085-472210.13057/biodiv/d210138https://doaj.org/article/10847ec77bdb4cb5bac72189c83bfeeb2019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4695https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play important roles in the fish protection system against bacterial infection. In this present study, we aimed to identify the single nucleotide polymorphism (SNP) within the LYSC gene of African catfish Clarias gariepinus and its possible association with Aeromonas hydrophila resistance, a major pathogenic bacterium in African catfish. The gDNA of the African catfish LYSC coding sequence was 1559 bp long, comprising of four exons and three introns. Six SNPs were identified in African catfish LYSC, namely SNP1-6. After the A. hydrophila challenge, we regarded the surviving individuals after the infection as the resistant group and the dead fish as the susceptible group. High-resolution melting (HRM) analysis on SNP2 revealed that the allele frequencies of TT, CC, and TC were of 27.78%, 5.55%, and 66.67% in the resistant group, while the frequencies of TT, CC, and TC were 16.67%, 27.78%, and 55.55% in the susceptible group, respectively. The expression of LYSC and other immune-genes in the resistant group was also higher in the liver, head kidney, and spleen. These results indicated that the LYSC gene might play an essential role in bacterial resistance, and the SNP2 within the LYSC gene may be associated with the resistance to A. hydrophila in African catfish.Hasan NasrullahYanti Inneke NababanIka SafitriDwi YantiSri NuryatiMuhammad Zairin JrAlimuddin AlimuddinMBI & UNS Soloarticleaeromonas hydrophila, african catfish, disease resistance, lysozyme gene, snpBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	aeromonas hydrophila, african catfish, disease resistance, lysozyme gene, snp Biology (General) QH301-705.5
spellingShingle	aeromonas hydrophila, african catfish, disease resistance, lysozyme gene, snp Biology (General) QH301-705.5 Hasan Nasrullah Yanti Inneke Nababan Ika Safitri Dwi Yanti Sri Nuryati Muhammad Zairin Jr Alimuddin Alimuddin Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
description	Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play important roles in the fish protection system against bacterial infection. In this present study, we aimed to identify the single nucleotide polymorphism (SNP) within the LYSC gene of African catfish Clarias gariepinus and its possible association with Aeromonas hydrophila resistance, a major pathogenic bacterium in African catfish. The gDNA of the African catfish LYSC coding sequence was 1559 bp long, comprising of four exons and three introns. Six SNPs were identified in African catfish LYSC, namely SNP1-6. After the A. hydrophila challenge, we regarded the surviving individuals after the infection as the resistant group and the dead fish as the susceptible group. High-resolution melting (HRM) analysis on SNP2 revealed that the allele frequencies of TT, CC, and TC were of 27.78%, 5.55%, and 66.67% in the resistant group, while the frequencies of TT, CC, and TC were 16.67%, 27.78%, and 55.55% in the susceptible group, respectively. The expression of LYSC and other immune-genes in the resistant group was also higher in the liver, head kidney, and spleen. These results indicated that the LYSC gene might play an essential role in bacterial resistance, and the SNP2 within the LYSC gene may be associated with the resistance to A. hydrophila in African catfish.
format	article
author	Hasan Nasrullah Yanti Inneke Nababan Ika Safitri Dwi Yanti Sri Nuryati Muhammad Zairin Jr Alimuddin Alimuddin
author_facet	Hasan Nasrullah Yanti Inneke Nababan Ika Safitri Dwi Yanti Sri Nuryati Muhammad Zairin Jr Alimuddin Alimuddin
author_sort	Hasan Nasrullah
title	Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
title_short	Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
title_full	Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
title_fullStr	Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
title_full_unstemmed	Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus
title_sort	short communication: single nucleotide polymorphism in c-type lysozyme gene and its correlation with aeromonas hydrophila resistance in african catfish clarias gariepinus
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/10847ec77bdb4cb5bac72189c83bfeeb
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Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

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