Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms

Abstract In this study, a hybrid magnetic-DNA directed immobilisation approach is presented to enhance protein capture and detection on a microfluidic platform. DNA-modified magnetic nanoparticles are added in a solution to capture fluorescently labelled immunocomplexes to be detected optically. A m...

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Autores principales:	Elaheh Esmaeili, Mohammad Adel Ghiass, Manouchehr Vossoughi, Masoud Soleimani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/108d06b147f84db9a48397e7ee1b628d
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spelling	oai:doaj.org-article:108d06b147f84db9a48397e7ee1b628d2021-12-02T15:04:58ZHybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms10.1038/s41598-017-00268-82045-2322https://doaj.org/article/108d06b147f84db9a48397e7ee1b628d2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00268-8https://doaj.org/toc/2045-2322Abstract In this study, a hybrid magnetic-DNA directed immobilisation approach is presented to enhance protein capture and detection on a microfluidic platform. DNA-modified magnetic nanoparticles are added in a solution to capture fluorescently labelled immunocomplexes to be detected optically. A magnetic set-up composed of cubic permanent magnets and a microchannel was designed and implemented based on finite element analysis results to efficiently concentrate the nanoparticles only over a defined area of the microchannel as the sensing zone. This in turn, led to the fluorescence emission localisation and the searching area reduction. Also, compared to processes in which the immunocomplex is formed directly on the surface, the proposed approach provides a lower steric hindrance, higher mass transfer, lower equilibrium time, and more surface concentration of the captured targets leading to a faster and more sensitive detection. As a proof-of-concept, the set-up is capable of detecting prostate-specific membrane antigen with concentrations down to 0.7 nM. Our findings suggest that the approach holds a great promise for applications in clinical assays and disease diagnosis.Elaheh EsmaeiliMohammad Adel GhiassManouchehr VossoughiMasoud SoleimaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elaheh Esmaeili Mohammad Adel Ghiass Manouchehr Vossoughi Masoud Soleimani Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
description	Abstract In this study, a hybrid magnetic-DNA directed immobilisation approach is presented to enhance protein capture and detection on a microfluidic platform. DNA-modified magnetic nanoparticles are added in a solution to capture fluorescently labelled immunocomplexes to be detected optically. A magnetic set-up composed of cubic permanent magnets and a microchannel was designed and implemented based on finite element analysis results to efficiently concentrate the nanoparticles only over a defined area of the microchannel as the sensing zone. This in turn, led to the fluorescence emission localisation and the searching area reduction. Also, compared to processes in which the immunocomplex is formed directly on the surface, the proposed approach provides a lower steric hindrance, higher mass transfer, lower equilibrium time, and more surface concentration of the captured targets leading to a faster and more sensitive detection. As a proof-of-concept, the set-up is capable of detecting prostate-specific membrane antigen with concentrations down to 0.7 nM. Our findings suggest that the approach holds a great promise for applications in clinical assays and disease diagnosis.
format	article
author	Elaheh Esmaeili Mohammad Adel Ghiass Manouchehr Vossoughi Masoud Soleimani
author_facet	Elaheh Esmaeili Mohammad Adel Ghiass Manouchehr Vossoughi Masoud Soleimani
author_sort	Elaheh Esmaeili
title	Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
title_short	Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
title_full	Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
title_fullStr	Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
title_full_unstemmed	Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms
title_sort	hybrid magnetic-dna directed immobilisation approach for efficient protein capture and detection on microfluidic platforms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/108d06b147f84db9a48397e7ee1b628d
work_keys_str_mv	AT elahehesmaeili hybridmagneticdnadirectedimmobilisationapproachforefficientproteincaptureanddetectiononmicrofluidicplatforms AT mohammadadelghiass hybridmagneticdnadirectedimmobilisationapproachforefficientproteincaptureanddetectiononmicrofluidicplatforms AT manouchehrvossoughi hybridmagneticdnadirectedimmobilisationapproachforefficientproteincaptureanddetectiononmicrofluidicplatforms AT masoudsoleimani hybridmagneticdnadirectedimmobilisationapproachforefficientproteincaptureanddetectiononmicrofluidicplatforms
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Hybrid Magnetic-DNA Directed Immobilisation Approach for Efficient Protein Capture and Detection on Microfluidic Platforms

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