Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling

Osteoporosis is characterized by the deterioration of bone structures and decreased bone mass, leading to an increased risk of fracture. Estrogen deficiency in postmenopausal women and aging are major factors of osteoporosis and are some of the reasons for reduced quality of life. In this study, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyung Jin Lim, Eun-Jae Park, Yeong-Seon Won, Seon Gyeong Bak, Sun Hee Cheong, Seung Woong Lee, Soyoung Lee, Seung-Jae Lee, Mun-Chual Rho
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>n-trans</i>-hibiscusamide 4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N-</i>(<i>E</i>)-hibiscusamide osteoclastogenesis OVX induced osteoporosis Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/1096cd9a3fea47bcb2254698825644be
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1096cd9a3fea47bcb2254698825644be
record_format	dspace
spelling	oai:doaj.org-article:1096cd9a3fea47bcb2254698825644be2021-11-25T18:27:28ZAnti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling10.3390/molecules262268201420-3049https://doaj.org/article/1096cd9a3fea47bcb2254698825644be2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6820https://doaj.org/toc/1420-3049Osteoporosis is characterized by the deterioration of bone structures and decreased bone mass, leading to an increased risk of fracture. Estrogen deficiency in postmenopausal women and aging are major factors of osteoporosis and are some of the reasons for reduced quality of life. In this study, we investigated the effects of <i>n-trans</i>-hibiscusamide (NHA) and its derivative 4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N</i>-(<i>E</i>)-hibiscusamide (HAD) on receptor activator of nuclear factor kappa-Β (NF-κB) ligand (RANKL)-induced osteoclast differentiation and an ovariectomized osteoporosis mouse model. NHA and HAD significantly inhibited the differentiation of osteoclasts from bone marrow-derived macrophages (BMMs) and the expression of osteoclast differentiation-related genes. At the molecular level, NHA and HAD significantly downregulated the phosphorylation of mitogen-activated protein kinase (MAPK) signaling molecules. However, Akt and NF-κB phosphorylation was inhibited only after NHA or HAD treatment. In the ovariectomy (OVX)-induced osteoporosis model, both NHA and HAD effectively improved trabecular bone structure. C-terminal telopeptide (CTX), a bone resorption marker, and RANKL, an osteoclast stimulation factor, were significantly reduced by NHA and HAD. The tartrate-resistant acid phosphatase (TRAP)-stained area, which indicates the osteoclast area, was also decreased by these compounds. These results show the potential of NHA and HAD as therapeutic agents for osteoporosis.Hyung Jin LimEun-Jae ParkYeong-Seon WonSeon Gyeong BakSun Hee CheongSeung Woong LeeSoyoung LeeSeung-Jae LeeMun-Chual RhoMDPI AGarticle<i>n-trans</i>-hibiscusamide4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N-</i>(<i>E</i>)-hibiscusamideosteoclastogenesisOVX induced osteoporosisOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6820, p 6820 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>n-trans</i>-hibiscusamide 4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N-</i>(<i>E</i>)-hibiscusamide osteoclastogenesis OVX induced osteoporosis Organic chemistry QD241-441
spellingShingle	<i>n-trans</i>-hibiscusamide 4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N-</i>(<i>E</i>)-hibiscusamide osteoclastogenesis OVX induced osteoporosis Organic chemistry QD241-441 Hyung Jin Lim Eun-Jae Park Yeong-Seon Won Seon Gyeong Bak Sun Hee Cheong Seung Woong Lee Soyoung Lee Seung-Jae Lee Mun-Chual Rho Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
description	Osteoporosis is characterized by the deterioration of bone structures and decreased bone mass, leading to an increased risk of fracture. Estrogen deficiency in postmenopausal women and aging are major factors of osteoporosis and are some of the reasons for reduced quality of life. In this study, we investigated the effects of <i>n-trans</i>-hibiscusamide (NHA) and its derivative 4-<i>O</i>-(<i>E</i>)-feruloyl-<i>N</i>-(<i>E</i>)-hibiscusamide (HAD) on receptor activator of nuclear factor kappa-Β (NF-κB) ligand (RANKL)-induced osteoclast differentiation and an ovariectomized osteoporosis mouse model. NHA and HAD significantly inhibited the differentiation of osteoclasts from bone marrow-derived macrophages (BMMs) and the expression of osteoclast differentiation-related genes. At the molecular level, NHA and HAD significantly downregulated the phosphorylation of mitogen-activated protein kinase (MAPK) signaling molecules. However, Akt and NF-κB phosphorylation was inhibited only after NHA or HAD treatment. In the ovariectomy (OVX)-induced osteoporosis model, both NHA and HAD effectively improved trabecular bone structure. C-terminal telopeptide (CTX), a bone resorption marker, and RANKL, an osteoclast stimulation factor, were significantly reduced by NHA and HAD. The tartrate-resistant acid phosphatase (TRAP)-stained area, which indicates the osteoclast area, was also decreased by these compounds. These results show the potential of NHA and HAD as therapeutic agents for osteoporosis.
format	article
author	Hyung Jin Lim Eun-Jae Park Yeong-Seon Won Seon Gyeong Bak Sun Hee Cheong Seung Woong Lee Soyoung Lee Seung-Jae Lee Mun-Chual Rho
author_facet	Hyung Jin Lim Eun-Jae Park Yeong-Seon Won Seon Gyeong Bak Sun Hee Cheong Seung Woong Lee Soyoung Lee Seung-Jae Lee Mun-Chual Rho
author_sort	Hyung Jin Lim
title	Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
title_short	Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
title_full	Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
title_fullStr	Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
title_full_unstemmed	Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling
title_sort	anti-osteoporotic effects of <i>n-trans</i>-hibiscusamide and its derivative alleviate ovariectomy-induced bone loss in mice by regulating rankl-induced signaling
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1096cd9a3fea47bcb2254698825644be
work_keys_str_mv	AT hyungjinlim antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT eunjaepark antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT yeongseonwon antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT seongyeongbak antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT sunheecheong antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT seungwoonglee antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT soyounglee antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT seungjaelee antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling AT munchualrho antiosteoporoticeffectsofintransihibiscusamideanditsderivativealleviateovariectomyinducedbonelossinmicebyregulatingranklinducedsignaling
_version_	1718411117178388480

Anti-Osteoporotic Effects of <i>n-trans</i>-Hibiscusamide and Its Derivative Alleviate Ovariectomy-Induced Bone Loss in Mice by Regulating RANKL-Induced Signaling

Ejemplares similares