Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.

COVID-19 testing is required before admission of a patient in the hospitals, invasive procedures, major and minor surgeries etc. Real Time Polymerase chain reaction is the gold standard test for the diagnosis, but requires well equipped biosafety laboratory along with trained manpower. In this study...

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Autores principales:	Ujjala Ghoshal, Atul Garg, Shruthi Vasanth, Akshay K Arya, Ankita Pandey, Nidhi Tejan, Vikas Patel, Vikram P Singh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1098ab32d31441969ad4ae535da8e052
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spelling	oai:doaj.org-article:1098ab32d31441969ad4ae535da8e0522021-12-02T20:07:54ZAssessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.1932-620310.1371/journal.pone.0257834https://doaj.org/article/1098ab32d31441969ad4ae535da8e0522021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257834https://doaj.org/toc/1932-6203COVID-19 testing is required before admission of a patient in the hospitals, invasive procedures, major and minor surgeries etc. Real Time Polymerase chain reaction is the gold standard test for the diagnosis, but requires well equipped biosafety laboratory along with trained manpower. In this study we have evaluated the diagnostic accuracy of novel TrueNat molecular assay for detecting SARS CoV-2. TrueNat is a chip-based real time PCR test and works on portable, light weight, battery powered equipment and can be used in remote areas with poor infrastructure. In this study 1807 patients samples were collected for both TrueNat and RTPCR COVID-19 testing during study period. Of these 174 (9.7%) and 174 (15%) were positive by RTPCR and TrueNat respectively and taking results of RTPCR as gold standard TrueNat test showed a sensitivity, specificity and diagnostic accuracy of 69.5, 90.9% and 89.2% respectively. It can be concluded that TrueNat is a simple, easy to use, good rapid molecular diagnostic test for diagnosis of COVID-19 especially in resource limited settings and will prove to be a game changer of molecular diagnostics in future.Ujjala GhoshalAtul GargShruthi VasanthAkshay K AryaAnkita PandeyNidhi TejanVikas PatelVikram P SinghPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257834 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ujjala Ghoshal Atul Garg Shruthi Vasanth Akshay K Arya Ankita Pandey Nidhi Tejan Vikas Patel Vikram P Singh Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
description	COVID-19 testing is required before admission of a patient in the hospitals, invasive procedures, major and minor surgeries etc. Real Time Polymerase chain reaction is the gold standard test for the diagnosis, but requires well equipped biosafety laboratory along with trained manpower. In this study we have evaluated the diagnostic accuracy of novel TrueNat molecular assay for detecting SARS CoV-2. TrueNat is a chip-based real time PCR test and works on portable, light weight, battery powered equipment and can be used in remote areas with poor infrastructure. In this study 1807 patients samples were collected for both TrueNat and RTPCR COVID-19 testing during study period. Of these 174 (9.7%) and 174 (15%) were positive by RTPCR and TrueNat respectively and taking results of RTPCR as gold standard TrueNat test showed a sensitivity, specificity and diagnostic accuracy of 69.5, 90.9% and 89.2% respectively. It can be concluded that TrueNat is a simple, easy to use, good rapid molecular diagnostic test for diagnosis of COVID-19 especially in resource limited settings and will prove to be a game changer of molecular diagnostics in future.
format	article
author	Ujjala Ghoshal Atul Garg Shruthi Vasanth Akshay K Arya Ankita Pandey Nidhi Tejan Vikas Patel Vikram P Singh
author_facet	Ujjala Ghoshal Atul Garg Shruthi Vasanth Akshay K Arya Ankita Pandey Nidhi Tejan Vikas Patel Vikram P Singh
author_sort	Ujjala Ghoshal
title	Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
title_short	Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
title_full	Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
title_fullStr	Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
title_full_unstemmed	Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
title_sort	assessing a chip based rapid rtpcr test for sars cov-2 detection (truenat assay): a diagnostic accuracy study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1098ab32d31441969ad4ae535da8e052
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Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.

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