Screening for candidate genes related to breast cancer with cDNA microarray analysis

Objective: The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease. Methods: We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tiss...

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Autores principales:	Yu-Juan Xiang, Qin-Ye Fu, Zhong-Bing Ma, De-Zong Gao, Qiang Zhang, Yu-Yang Li, Liang Li, Lu Liu, Chun-Miao Ye, Zhi-Gang Yu, Ming-Ming Guo
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/109b9023a37940d8b4b20d3294c0fbe2
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spelling	oai:doaj.org-article:109b9023a37940d8b4b20d3294c0fbe22021-12-02T13:36:53ZScreening for candidate genes related to breast cancer with cDNA microarray analysis2095-882X10.1016/j.cdtm.2015.02.001https://doaj.org/article/109b9023a37940d8b4b20d3294c0fbe22015-06-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095882X1500002Xhttps://doaj.org/toc/2095-882XObjective: The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease. Methods: We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tissues as controls using the cDNA microarray analysis in seven breast cancer patients. Further, one representative gene, named IFI30, was quantitatively analyzed by real-time PCR to confirm the result of the cDNA microarray analysis. Results: A total of 427 genes were identified with significantly differential expression, 221 genes were up-regulated and 206 genes were down-regulated. And the result of cDNA microarray analysis was validated by detection of IFI30 mRNA level changes by real-time PCR. Genes for cell proliferation, cell cycle, cell division, mitosis, apoptosis, and immune response were enriched in the up-regulated genes, while genes for cell adhesion, proteolysis, and transport were significantly enriched in the down-regulated genes in breast cancer tissues compared with normal breast tissues by a gene ontology analysis. Conclusion: Our present study revealed a range of differentially expressed genes between breast cancer tissues and normal breast tissues, and provide candidate genes for further study focusing on the pathogenesis and new biomarkers for breast cancer. Keywords: Breast neoplasms, Candidate genes, MicroarrayYu-Juan XiangQin-Ye FuZhong-Bing MaDe-Zong GaoQiang ZhangYu-Yang LiLiang LiLu LiuChun-Miao YeZhi-Gang YuMing-Ming GuoKeAi Communications Co., Ltd.articleMedicine (General)R5-920ENChronic Diseases and Translational Medicine, Vol 1, Iss 2, Pp 65-72 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yu-Juan Xiang Qin-Ye Fu Zhong-Bing Ma De-Zong Gao Qiang Zhang Yu-Yang Li Liang Li Lu Liu Chun-Miao Ye Zhi-Gang Yu Ming-Ming Guo Screening for candidate genes related to breast cancer with cDNA microarray analysis
description	Objective: The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease. Methods: We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tissues as controls using the cDNA microarray analysis in seven breast cancer patients. Further, one representative gene, named IFI30, was quantitatively analyzed by real-time PCR to confirm the result of the cDNA microarray analysis. Results: A total of 427 genes were identified with significantly differential expression, 221 genes were up-regulated and 206 genes were down-regulated. And the result of cDNA microarray analysis was validated by detection of IFI30 mRNA level changes by real-time PCR. Genes for cell proliferation, cell cycle, cell division, mitosis, apoptosis, and immune response were enriched in the up-regulated genes, while genes for cell adhesion, proteolysis, and transport were significantly enriched in the down-regulated genes in breast cancer tissues compared with normal breast tissues by a gene ontology analysis. Conclusion: Our present study revealed a range of differentially expressed genes between breast cancer tissues and normal breast tissues, and provide candidate genes for further study focusing on the pathogenesis and new biomarkers for breast cancer. Keywords: Breast neoplasms, Candidate genes, Microarray
format	article
author	Yu-Juan Xiang Qin-Ye Fu Zhong-Bing Ma De-Zong Gao Qiang Zhang Yu-Yang Li Liang Li Lu Liu Chun-Miao Ye Zhi-Gang Yu Ming-Ming Guo
author_facet	Yu-Juan Xiang Qin-Ye Fu Zhong-Bing Ma De-Zong Gao Qiang Zhang Yu-Yang Li Liang Li Lu Liu Chun-Miao Ye Zhi-Gang Yu Ming-Ming Guo
author_sort	Yu-Juan Xiang
title	Screening for candidate genes related to breast cancer with cDNA microarray analysis
title_short	Screening for candidate genes related to breast cancer with cDNA microarray analysis
title_full	Screening for candidate genes related to breast cancer with cDNA microarray analysis
title_fullStr	Screening for candidate genes related to breast cancer with cDNA microarray analysis
title_full_unstemmed	Screening for candidate genes related to breast cancer with cDNA microarray analysis
title_sort	screening for candidate genes related to breast cancer with cdna microarray analysis
publisher	KeAi Communications Co., Ltd.
publishDate	2015
url	https://doaj.org/article/109b9023a37940d8b4b20d3294c0fbe2
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_version_	1718392658123030528

Screening for candidate genes related to breast cancer with cDNA microarray analysis

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