Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.

<h4>Purpose</h4>The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls.<h4>Methods</h4>This prospective study included all infants <1 year with microbiologically confirmed di...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muntsa Rocafort, Desiree Henares, Pedro Brotons, Irene Barrabeig, Cristian Launes, Lore Merdrignac, Marta Valenciano, Angela Domínguez, Pere Godoy, Carmen Muñoz-Almagro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/109ce1c63fe7446eac2c787de119a243
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:109ce1c63fe7446eac2c787de119a243
record_format	dspace
spelling	oai:doaj.org-article:109ce1c63fe7446eac2c787de119a2432021-12-02T20:13:18ZExploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.1932-620310.1371/journal.pone.0259318https://doaj.org/article/109ce1c63fe7446eac2c787de119a2432021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259318https://doaj.org/toc/1932-6203<h4>Purpose</h4>The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls.<h4>Methods</h4>This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR.<h4>Results</h4>Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy control.<h4>Conclusion</h4>This study supports the usefulness of 16S rRNA gene sequencing for improved sensitivity on pertussis diagnosis compared to real-time PCR and to understand other microbial changes occurring in the nasopharynx in children <1 year old with suspected whooping cough compared to healthy controls.Muntsa RocafortDesiree HenaresPedro BrotonsIrene BarrabeigCristian LaunesLore MerdrignacMarta ValencianoAngela DomínguezPere GodoyCarmen Muñoz-AlmagroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0259318 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
description	<h4>Purpose</h4>The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls.<h4>Methods</h4>This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR.<h4>Results</h4>Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy control.<h4>Conclusion</h4>This study supports the usefulness of 16S rRNA gene sequencing for improved sensitivity on pertussis diagnosis compared to real-time PCR and to understand other microbial changes occurring in the nasopharynx in children <1 year old with suspected whooping cough compared to healthy controls.
format	article
author	Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro
author_facet	Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro
author_sort	Muntsa Rocafort
title	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
title_short	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
title_full	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
title_fullStr	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
title_full_unstemmed	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.
title_sort	exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/109ce1c63fe7446eac2c787de119a243
work_keys_str_mv	AT muntsarocafort exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT desireehenares exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT pedrobrotons exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT irenebarrabeig exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT cristianlaunes exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT loremerdrignac exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT martavalenciano exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT angeladominguez exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT peregodoy exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy AT carmenmunozalmagro exploringthenasopharyngealmicrobiotacompositionininfantswithwhoopingcoughatestnegativecasecontrolstudy
_version_	1718374748815097856

Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study.

Ejemplares similares