Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.

We present a novel study on label-free recognition and distinction of drug resistant breast cancer cells (MCF-7 DOX) from their parental cells (MCF-7 WT) via impedimetric measurements. Drug resistant cells exhibited significant differences in their dielectric properties compared to wild-type cells,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bilge Eker, Robert Meissner, Arnaud Bertsch, Kapil Mehta, Philippe Renaud
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10a2344748ef4aa8880a3e7a5eb47a01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:10a2344748ef4aa8880a3e7a5eb47a01
record_format	dspace
spelling	oai:doaj.org-article:10a2344748ef4aa8880a3e7a5eb47a012021-11-18T07:55:04ZLabel-free recognition of drug resistance via impedimetric screening of breast cancer cells.1932-620310.1371/journal.pone.0057423https://doaj.org/article/10a2344748ef4aa8880a3e7a5eb47a012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483910/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We present a novel study on label-free recognition and distinction of drug resistant breast cancer cells (MCF-7 DOX) from their parental cells (MCF-7 WT) via impedimetric measurements. Drug resistant cells exhibited significant differences in their dielectric properties compared to wild-type cells, exerting much higher extracellular resistance (Rextra ). Immunostaining revealed that MCF-7 DOX cells gained a much denser F-actin network upon acquiring drug resistance indicating that remodeling of actin cytoskeleton is probably the reason behind higher Rextra , providing stronger cell architecture. Moreover, having exposed both cell types to doxorubicin, we were able to distinguish these two phenotypes based on their substantially different drug response. Interestingly, impedimetric measurements identified a concentration-dependent and reversible increase in cell stiffness in the presence of low non-lethal drug doses. Combined with a profound frequency analysis, these findings enabled distinguishing distinct cellular responses during drug exposure within four concentration ranges without using any labeling. Overall, this study highlights the possibility to differentiate drug resistant phenotypes from their parental cells and to assess their drug response by using microelectrodes, offering direct, real-time and noninvasive measurements of cell dependent parameters under drug exposure, hence providing a promising step for personalized medicine applications such as evaluation of the disease progress and optimization of the drug treatment of a patient during chemotherapy.Bilge EkerRobert MeissnerArnaud BertschKapil MehtaPhilippe RenaudPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57423 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bilge Eker Robert Meissner Arnaud Bertsch Kapil Mehta Philippe Renaud Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
description	We present a novel study on label-free recognition and distinction of drug resistant breast cancer cells (MCF-7 DOX) from their parental cells (MCF-7 WT) via impedimetric measurements. Drug resistant cells exhibited significant differences in their dielectric properties compared to wild-type cells, exerting much higher extracellular resistance (Rextra ). Immunostaining revealed that MCF-7 DOX cells gained a much denser F-actin network upon acquiring drug resistance indicating that remodeling of actin cytoskeleton is probably the reason behind higher Rextra , providing stronger cell architecture. Moreover, having exposed both cell types to doxorubicin, we were able to distinguish these two phenotypes based on their substantially different drug response. Interestingly, impedimetric measurements identified a concentration-dependent and reversible increase in cell stiffness in the presence of low non-lethal drug doses. Combined with a profound frequency analysis, these findings enabled distinguishing distinct cellular responses during drug exposure within four concentration ranges without using any labeling. Overall, this study highlights the possibility to differentiate drug resistant phenotypes from their parental cells and to assess their drug response by using microelectrodes, offering direct, real-time and noninvasive measurements of cell dependent parameters under drug exposure, hence providing a promising step for personalized medicine applications such as evaluation of the disease progress and optimization of the drug treatment of a patient during chemotherapy.
format	article
author	Bilge Eker Robert Meissner Arnaud Bertsch Kapil Mehta Philippe Renaud
author_facet	Bilge Eker Robert Meissner Arnaud Bertsch Kapil Mehta Philippe Renaud
author_sort	Bilge Eker
title	Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
title_short	Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
title_full	Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
title_fullStr	Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
title_full_unstemmed	Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
title_sort	label-free recognition of drug resistance via impedimetric screening of breast cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/10a2344748ef4aa8880a3e7a5eb47a01
work_keys_str_mv	AT bilgeeker labelfreerecognitionofdrugresistanceviaimpedimetricscreeningofbreastcancercells AT robertmeissner labelfreerecognitionofdrugresistanceviaimpedimetricscreeningofbreastcancercells AT arnaudbertsch labelfreerecognitionofdrugresistanceviaimpedimetricscreeningofbreastcancercells AT kapilmehta labelfreerecognitionofdrugresistanceviaimpedimetricscreeningofbreastcancercells AT philipperenaud labelfreerecognitionofdrugresistanceviaimpedimetricscreeningofbreastcancercells
_version_	1718422711526490112

Label-free recognition of drug resistance via impedimetric screening of breast cancer cells.

Ejemplares similares