Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods

Tamara Fernandez Cabada1,2,*, Cristina Sanchez Lopez de Pablo1,3,*, Alberto Martinez Serrano2, Francisco del Pozo Guerrero1,3, Jose Javier Serrano Olmedo1,3,*, Milagros Ramos Gomez1&ndash;3,* 1Centre for Biomedical Technology, Universidad Politecnica de Madrid, Madrid, Spain; 2Centre for Mol...

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Autores principales:	Fernandez Cabada T, Sanchez Lopez de Pablo C, Martinez Serrano A, del Pozo Guerrero F, Serrano Olmedo JJ, Ramos Gomez M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/10a24844ab874ba3bf0b9107415589c4
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spelling	oai:doaj.org-article:10a24844ab874ba3bf0b9107415589c42021-12-02T02:16:49ZInduction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods1176-91141178-2013https://doaj.org/article/10a24844ab874ba3bf0b9107415589c42012-03-01T00:00:00Zhttp://www.dovepress.com/induction-of-cell-death-in-a-glioblastoma-line-by-hyperthermic-therapy-a9533https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tamara Fernandez Cabada1,2,, Cristina Sanchez Lopez de Pablo1,3,, Alberto Martinez Serrano2, Francisco del Pozo Guerrero1,3, Jose Javier Serrano Olmedo1,3,, Milagros Ramos Gomez1&ndash;3, 1Centre for Biomedical Technology, Universidad Politecnica de Madrid, Madrid, Spain; 2Centre for Molecular Biology, &quot;Severo Ochoa&quot; Universidad Autonoma de Madrid, Madrid, Spain; 3Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER-bbn), Zaragoza, Spain.*These authors contributed equally to this workBackground: Metallic nanorods are promising agents for a wide range of biomedical applications. In this study, we developed an optical hyperthermia method capable of inducing in vitro death of glioblastoma cells.Methods: The procedure used was based on irradiation of gold nanorods with a continuous wave laser. This kind of nanoparticle converts absorbed light into localized heat within a short period of time due to the surface plasmon resonance effect. The effectiveness of the method was determined by measuring changes in cell viability after laser irradiation of glioblastoma cells in the presence of gold nanorods.Results: Laser irradiation in the presence of gold nanorods induced a significant decrease in cell viability, while no decrease in cell viability was observed with laser irradiation or incubation with gold nanorods alone. The mechanism of cell death mediated by gold nanorods during photothermal ablation was analyzed, indicating that treatment compromised the integrity of the cell membrane instead of initiating the process of programmed cell death.Conclusion: The use of gold nanorods in hyperthermal therapies is very effective in eliminating glioblastoma cells, and therefore represents an important area of research for therapeutic development.Keywords: laser irradiation, photothermal therapy, surface plasmon resonance, cancerFernandez Cabada TSanchez Lopez de Pablo CMartinez Serrano Adel Pozo Guerrero FSerrano Olmedo JJRamos Gomez MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1511-1523 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Fernandez Cabada T Sanchez Lopez de Pablo C Martinez Serrano A del Pozo Guerrero F Serrano Olmedo JJ Ramos Gomez M Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
description	Tamara Fernandez Cabada1,2,, Cristina Sanchez Lopez de Pablo1,3,, Alberto Martinez Serrano2, Francisco del Pozo Guerrero1,3, Jose Javier Serrano Olmedo1,3,, Milagros Ramos Gomez1&ndash;3, 1Centre for Biomedical Technology, Universidad Politecnica de Madrid, Madrid, Spain; 2Centre for Molecular Biology, &quot;Severo Ochoa&quot; Universidad Autonoma de Madrid, Madrid, Spain; 3Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER-bbn), Zaragoza, Spain.*These authors contributed equally to this workBackground: Metallic nanorods are promising agents for a wide range of biomedical applications. In this study, we developed an optical hyperthermia method capable of inducing in vitro death of glioblastoma cells.Methods: The procedure used was based on irradiation of gold nanorods with a continuous wave laser. This kind of nanoparticle converts absorbed light into localized heat within a short period of time due to the surface plasmon resonance effect. The effectiveness of the method was determined by measuring changes in cell viability after laser irradiation of glioblastoma cells in the presence of gold nanorods.Results: Laser irradiation in the presence of gold nanorods induced a significant decrease in cell viability, while no decrease in cell viability was observed with laser irradiation or incubation with gold nanorods alone. The mechanism of cell death mediated by gold nanorods during photothermal ablation was analyzed, indicating that treatment compromised the integrity of the cell membrane instead of initiating the process of programmed cell death.Conclusion: The use of gold nanorods in hyperthermal therapies is very effective in eliminating glioblastoma cells, and therefore represents an important area of research for therapeutic development.Keywords: laser irradiation, photothermal therapy, surface plasmon resonance, cancer
format	article
author	Fernandez Cabada T Sanchez Lopez de Pablo C Martinez Serrano A del Pozo Guerrero F Serrano Olmedo JJ Ramos Gomez M
author_facet	Fernandez Cabada T Sanchez Lopez de Pablo C Martinez Serrano A del Pozo Guerrero F Serrano Olmedo JJ Ramos Gomez M
author_sort	Fernandez Cabada T
title	Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
title_short	Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
title_full	Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
title_fullStr	Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
title_full_unstemmed	Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
title_sort	induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/10a24844ab874ba3bf0b9107415589c4
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Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods

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