Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae

Spread of the carbapenemase-encoding and mobilized colistin resistance (mcr) genes among Enterobacteriales poses a great threat to global public health, especially when the both genes are transferred by a single plasmid. Here, we identified a blaNDM–1- and mcr-9-co-encoding plasmid harbored by a cli...

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Autores principales:	Zhou Liu, Xiubin Hang, Xiao Xiao, Wenwen Chu, Xin Li, Yangyang Liu, Xi Li, Qiang Zhou, Jiabin Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Klebsiella pneumoniae carbapenem-resistant blaNDM–1 mcr-9 IncHI2 plasmid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/10a25136d3a74cda9150c94589b3b57f
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spelling	oai:doaj.org-article:10a25136d3a74cda9150c94589b3b57f2021-12-01T18:39:41ZCo-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae1664-302X10.3389/fmicb.2021.756201https://doaj.org/article/10a25136d3a74cda9150c94589b3b57f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.756201/fullhttps://doaj.org/toc/1664-302XSpread of the carbapenemase-encoding and mobilized colistin resistance (mcr) genes among Enterobacteriales poses a great threat to global public health, especially when the both genes are transferred by a single plasmid. Here, we identified a blaNDM–1- and mcr-9-co-encoding plasmid harbored by a clinical isolate of Klebsiella pneumoniae (KPN710429). KPN710429 was recovered from a blood sample from an inpatient in a tertiary hospital in China, and antimicrobial susceptibility testing showed that it was multidrug-resistant and only susceptible to aztreonam, colistin, and tigecycline. KPN710429 belongs to sequence type (ST) 1308 and capsular serotype KL144. The string test of KPN710429 was negative, and this strain didn’t exhibit a hypervirulent phenotype according to serum-killing and Galleria mellonella lethality assessments. Whole-genome sequencing revealed the KPN710429 genome comprises a single chromosome and three plasmids. All virulence associated genes were harbored by chromosome. Most of its antimicrobial resistance genes, including blaNDM–1 and mcr-9 were carried by plasmid pK701429_2, belonging to the incompatibility (Inc) HI2/HI2A group and ST1. Comparative genomics assays indicates that pK710429_2 could be a hybrid plasmid, formed by a Tn6696-like blaNDM–1 region inserting into a mcr-9-positive-IncHI2/HI2A plasmid. pK710429_2 contained the conjugative transfer gene regions, Tra1 and Tra2, with some structural variations, and conjugation assays revealed that pK710429_2 was transferable. Although pK710429_2 lacked the qseB-qseC regulatory genes, mcr-9 expression was upregulated after pretreatment with colistin for 6 h, leading to colistin resistance in KPN710429. To our knowledge, this is the first report of a blaNDM–1- and mcr-9-co-encoding transferable plasmid harbored by a bloodstream-infection-causing K. pneumoniae strain in China. Effective surveillance should be implemented to assess the prevalence of the plasmid co-harboring carbapenemase-encoding gene and mcr-9.Zhou LiuXiubin HangXiao XiaoWenwen ChuXin LiYangyang LiuYangyang LiuYangyang LiuXi LiQiang ZhouJiabin LiJiabin LiJiabin LiJiabin LiFrontiers Media S.A.articleKlebsiella pneumoniaecarbapenem-resistantblaNDM–1mcr-9IncHI2plasmidMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae carbapenem-resistant blaNDM–1 mcr-9 IncHI2 plasmid Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae carbapenem-resistant blaNDM–1 mcr-9 IncHI2 plasmid Microbiology QR1-502 Zhou Liu Xiubin Hang Xiao Xiao Wenwen Chu Xin Li Yangyang Liu Yangyang Liu Yangyang Liu Xi Li Qiang Zhou Jiabin Li Jiabin Li Jiabin Li Jiabin Li Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
description	Spread of the carbapenemase-encoding and mobilized colistin resistance (mcr) genes among Enterobacteriales poses a great threat to global public health, especially when the both genes are transferred by a single plasmid. Here, we identified a blaNDM–1- and mcr-9-co-encoding plasmid harbored by a clinical isolate of Klebsiella pneumoniae (KPN710429). KPN710429 was recovered from a blood sample from an inpatient in a tertiary hospital in China, and antimicrobial susceptibility testing showed that it was multidrug-resistant and only susceptible to aztreonam, colistin, and tigecycline. KPN710429 belongs to sequence type (ST) 1308 and capsular serotype KL144. The string test of KPN710429 was negative, and this strain didn’t exhibit a hypervirulent phenotype according to serum-killing and Galleria mellonella lethality assessments. Whole-genome sequencing revealed the KPN710429 genome comprises a single chromosome and three plasmids. All virulence associated genes were harbored by chromosome. Most of its antimicrobial resistance genes, including blaNDM–1 and mcr-9 were carried by plasmid pK701429_2, belonging to the incompatibility (Inc) HI2/HI2A group and ST1. Comparative genomics assays indicates that pK710429_2 could be a hybrid plasmid, formed by a Tn6696-like blaNDM–1 region inserting into a mcr-9-positive-IncHI2/HI2A plasmid. pK710429_2 contained the conjugative transfer gene regions, Tra1 and Tra2, with some structural variations, and conjugation assays revealed that pK710429_2 was transferable. Although pK710429_2 lacked the qseB-qseC regulatory genes, mcr-9 expression was upregulated after pretreatment with colistin for 6 h, leading to colistin resistance in KPN710429. To our knowledge, this is the first report of a blaNDM–1- and mcr-9-co-encoding transferable plasmid harbored by a bloodstream-infection-causing K. pneumoniae strain in China. Effective surveillance should be implemented to assess the prevalence of the plasmid co-harboring carbapenemase-encoding gene and mcr-9.
format	article
author	Zhou Liu Xiubin Hang Xiao Xiao Wenwen Chu Xin Li Yangyang Liu Yangyang Liu Yangyang Liu Xi Li Qiang Zhou Jiabin Li Jiabin Li Jiabin Li Jiabin Li
author_facet	Zhou Liu Xiubin Hang Xiao Xiao Wenwen Chu Xin Li Yangyang Liu Yangyang Liu Yangyang Liu Xi Li Qiang Zhou Jiabin Li Jiabin Li Jiabin Li Jiabin Li
author_sort	Zhou Liu
title	Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
title_short	Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
title_full	Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
title_fullStr	Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
title_full_unstemmed	Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae
title_sort	co-occurrence of blandm–1 and mcr-9 in a conjugative inchi2/hi2a plasmid from a bloodstream infection-causing carbapenem-resistant klebsiella pneumoniae
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/10a25136d3a74cda9150c94589b3b57f
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Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae

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