A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees

A revised protocol has been developed for in vitro propogation of papaya using explants from field-grown trees. Successful establishment of papaya in vitro using lateral buds could be obtained by treating the buds with Carbendazim (0.2%) and Streptomycin (0.1%) for 24h, followed by surface steriliza...

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Autores principales:	Prakash Patil, Neeta Vastrad, M R Dinesh, A R Bantwal
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2007
Materias:	micropropagation mature explants carica papaya Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/10a612add01b4d72886f00223d384d68
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spelling	oai:doaj.org-article:10a612add01b4d72886f00223d384d682021-12-02T04:01:57ZA Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees0973-354X2582-4899https://doaj.org/article/10a612add01b4d72886f00223d384d682007-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/613https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899A revised protocol has been developed for in vitro propogation of papaya using explants from field-grown trees. Successful establishment of papaya in vitro using lateral buds could be obtained by treating the buds with Carbendazim (0.2%) and Streptomycin (0.1%) for 24h, followed by surface sterilization with mercuric chloride (0.1%) for 3 minutes and culturing on MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Established buds were proliferated on modified MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Modified MS medium supplemented with BAP (0.3 mg/l), NAA (0.1 mg/l) and GA3 (1 mg/l) caused extensive elongation of shoots. Elongated shootlets were rooted on half-strength MS medium supplemented with BAP (0.1mg/l), NAA (0.1 mg/l) and IBA (2 mg/l). Rooted plantlets were initially hardened on a potting mixture consisting of soilrite and later on a mixture of sand, soil and FYM (1:1:1).Prakash PatilNeeta VastradM R DineshA R BantwalSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlemicropropagationmature explantscarica papayaPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 2, Iss 2, Pp 99-103 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	micropropagation mature explants carica papaya Plant culture SB1-1110
spellingShingle	micropropagation mature explants carica papaya Plant culture SB1-1110 Prakash Patil Neeta Vastrad M R Dinesh A R Bantwal A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
description	A revised protocol has been developed for in vitro propogation of papaya using explants from field-grown trees. Successful establishment of papaya in vitro using lateral buds could be obtained by treating the buds with Carbendazim (0.2%) and Streptomycin (0.1%) for 24h, followed by surface sterilization with mercuric chloride (0.1%) for 3 minutes and culturing on MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Established buds were proliferated on modified MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Modified MS medium supplemented with BAP (0.3 mg/l), NAA (0.1 mg/l) and GA3 (1 mg/l) caused extensive elongation of shoots. Elongated shootlets were rooted on half-strength MS medium supplemented with BAP (0.1mg/l), NAA (0.1 mg/l) and IBA (2 mg/l). Rooted plantlets were initially hardened on a potting mixture consisting of soilrite and later on a mixture of sand, soil and FYM (1:1:1).
format	article
author	Prakash Patil Neeta Vastrad M R Dinesh A R Bantwal
author_facet	Prakash Patil Neeta Vastrad M R Dinesh A R Bantwal
author_sort	Prakash Patil
title	A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
title_short	A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
title_full	A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
title_fullStr	A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
title_full_unstemmed	A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
title_sort	revised protocol for in vitro propagation of carica papaya using lateral buds from field-grown trees
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2007
url	https://doaj.org/article/10a612add01b4d72886f00223d384d68
work_keys_str_mv	AT prakashpatil arevisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT neetavastrad arevisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT mrdinesh arevisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT arbantwal arevisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT prakashpatil revisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT neetavastrad revisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT mrdinesh revisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees AT arbantwal revisedprotocolforinvitropropagationofcaricapapayausinglateralbudsfromfieldgrowntrees
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A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees

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